公开(公告)号：US20090221806A1

公开(公告)日：2009-09-03

申请号：US10544820

申请日：2004-03-04

申请人： Werner Frohnwieser ,  Michael Volland ,  Evi Wittmann ,  Fabienne Skorupinsui ,  Jean Jacques Lebehot ,  Yves Lemoigne ,  Thomas Lötzbeyer

发明人： Werner Frohnwieser ,  Michael Volland ,  Evi Wittmann ,  Fabienne Skorupinsui ,  Jean Jacques Lebehot ,  Yves Lemoigne ,  Thomas Lötzbeyer

IPC分类号： C07G3/00 ,  C12P19/44

CPC分类号： C12P19/04 ,  C08B37/0024 ,  C12P19/14

摘要： In this process for preparing a β-1,3-glucan, the glucan-containing matrix is treated with a protein having β(1,3)-glucanase activity, wherein the concentration of the glucanase amounts from 0.001 to 3.0% by weight. The glucan-containing matrix can be a fermentation broth, a culture medium or a suspension, where appropriate containing unsolved solids, cell constituents and/or cell fragments, or else a mycelium, a hydrocolloid or a powder preparation having a solvent proportion of from 20 to 99.9% by weight. The duration of the enzymatic treatment should be between 15 minutes and 24 hours and the treatment should be carried out continuously. The invention also envisages the glucan-containing matrix being filtered or centrifuged, after it has been treated enzymatically, and the glucan finally being separated off. A β-1,3-glucan which has been prepared in this way and which exhibits, for example, improved solubility in cold water, reduced proportions of insoluble constituents, an increased viscosity or improved filterability, is also claimed. A solid formulation and the use of these glucans for cosmetic applications, foodstuffs or oil production are also coclaimed.

摘要翻译： 在制备β-1,3-葡聚糖的该方法中，含有葡聚糖的基质用具有β（1,3） - 葡聚糖酶活性的蛋白质处理，其中葡聚糖酶的浓度为0.001至3.0重量％。 含有葡聚糖的基质可以是发酵液，培养基或悬浮液，其中适当地含有未溶解的固体，细胞成分和/或细胞碎片，或者是溶剂比例为20的菌丝体，水胶体或粉末制剂 至99.9重量％。 酶处理的持续时间应在15分钟至24小时之间，治疗应持续进行。 本发明还设想在经酶处理的葡聚糖的基质被过滤或离心后，最后分离出葡聚糖。 已经以这种方式制备并且例如显示出在冷水中的改善的溶解性，减少的不溶性组分的比例，增加的粘度或改进的过滤性的β-1,3-葡聚糖也被要求保护。 固体制剂和这些葡聚糖用于化妆品应用，食品或油品生产也被广泛使用。