公开(公告)号：US20060064769A1

公开(公告)日：2006-03-23

申请号：US11191157

申请日：2005-07-26

申请人： Stephen Jones ,  Zhinqing Zhu

发明人： Stephen Jones ,  Zhinqing Zhu

IPC分类号： A01K67/027 ,  C12N5/06 ,  C12N15/87

CPC分类号： C12N15/8509 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  C12N9/90 ,  C12N2800/30

摘要： The present invention is based, at least in part, on the generation of cells, cell lines and non-human mammals that contain a synthetic conditional allele of the Dicer1 gene. Many of the constructs, methods, animals and cells of the invention feature recombinase recognition sites (in certain embodiments, loxP sites) positioned on either flank of a sequence that comprises exons 14, 15 and 16 of the Dicer1 gene (in certain embodiments, the mouse Dicer1 gene). Through readily manipulable introduction, administration, or expression of a recombinase (in certain aspects of the invention, the Cre recombinase of bacteriophage P1), exons 14 through 16 of Dicer1 may be directedly excised, producing a functional knockout of Dicer1 in cells containing these recombinase-induced alleles of the invention.

摘要翻译： 本发明至少部分地基于含有Dicer1基因的合成条件等位基因的细胞，细胞系和非人哺乳动物的产生。 本发明的许多构建体，方法，动物和细胞的特征在于位于包含Dicer1基因的外显子14,15和16的序列的任一侧的重组酶识别位点（在某些实施方案中，loxP位点）（在某些实施方案中， 小鼠Dicer1基因）。 通过易于操作的引入，施用或表达重组酶（在本发明的某些方面，噬菌体P1的Cre重组酶），Dicer1的外显子14至16可以被直接切除，在含有这些重组酶的细胞中产生Dicer1的功能性敲除 诱导的本发明的等位基因。

公开(公告)号：US07582741B2

公开(公告)日：2009-09-01

申请号：US11191157

申请日：2005-07-26

申请人： Stephen Jones ,  Zhinqing Zhu

发明人： Stephen Jones ,  Zhinqing Zhu

IPC分类号： C12N15/11 ,  C12N15/74

CPC分类号： C12N15/8509 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  C12N9/90 ,  C12N2800/30

摘要： The present invention is based, at least in part, on the generation of cells, cell lines and non-human mammals that contain a synthetic conditional allele of the Dicer1 gene. Many of the constructs, methods, animals and cells of the invention feature recombinase recognition sites (in certain embodiments, loxP sites) positioned on either flank of a sequence that comprises exons 14, 15 and 16 of the Dicer1 gene (in certain embodiments, the mouse Dicer1 gene). Through readily manipulable introduction, administration, or expression of a recombinase (in certain aspects of the invention, the Cre recombinase of bacteriophage P1), exons 14 through 16 of Dicer1 may be directedly excised, producing a functional knockout of Dicer1 in cells containing these recombinase-induced alleles of the invention.

摘要翻译： 本发明至少部分地基于含有Dicer1基因的合成条件等位基因的细胞，细胞系和非人哺乳动物的产生。 本发明的许多构建体，方法，动物和细胞的特征在于位于包含Dicer1基因的外显子14,15和16的序列的任一侧的重组酶识别位点（在某些实施方案中，loxP位点）（在某些实施方案中， 小鼠Dicer1基因）。 通过易于操作的引入，施用或表达重组酶（在本发明的某些方面，噬菌体P1的Cre重组酶），Dicer1的外显子14至16可以被直接切除，在含有这些重组酶的细胞中产生Dicer1的功能性敲除 诱导的本发明的等位基因。