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公开(公告)号:US20200263232A1
公开(公告)日:2020-08-20
申请号:US16530930
申请日:2019-08-02
申请人: 10X GENOMICS, INC.
发明人: Jason Bell , Josephine Lee , Corey Nemec , Francesca Meschi
IPC分类号: C12Q1/6806 , C12N9/12 , C12N15/10
摘要: Methods and compositions to minimize barcode exchange during the preparation of barcoded next-generation sequencing libraries prepared from a single cell. The methods utilize oligonucleotides containing a 3′-terminated blocking group or sequences that prevent amplification or extension.