公开(公告)号：US11636921B2

公开(公告)日：2023-04-25

申请号：US16698612

申请日：2019-11-27

Applicant: 10X Genomics, Inc.

Inventor： Michael Schnall-Levin ,  Rajiv Bharadwaj ,  Zahra Kamila Belhocine ,  Andrew D. Price ,  Yifeng Yin ,  Vijay Kumar Sreenivasa Gopalan ,  Zeljko Jovan Dzakula

IPC: G16B30/00 ,  G16H10/40 ,  G16H20/00

Abstract: Systems and methods for inferring a status of a cell population are provided. Described techniques allow deconvolving a first clonal population comprising a first plurality of cells of a species, wherein nucleic acid sequence reads from each cell in the first plurality of cells are obtained. The nucleic acid sequence reads are mapped into bins representing portions of a reference genome, and a pattern of sequence read counts for each cell across the multiple bins is used to assign a cell to a group, thereby inferring a mitotic status of the cell. The assignment of nucleic acid sequence reads into bins is also be used for segregating cells into classes based on a status of a certain biological marker in each cell. Comparison of sequence read counts for a subset of bins across the cell classes allows evaluating effect of a compound on a cell status.

公开(公告)号：US20200168297A1

公开(公告)日：2020-05-28

申请号：US16698612

申请日：2019-11-27

Applicant: 10X Genomics, Inc.

Inventor： Michael Schnall-Levin ,  Rajiv Bharadwaj ,  Kamila Belhocine ,  Andrew D. Price ,  Yifeng Yin ,  Vijay Kumar Sreenivasa Gopalan ,  Zeljko Jovan Dzakula

IPC: G16B30/00 ,  G16H10/40 ,  G16H20/00

Abstract: Systems and methods for inferring a status of a cell population are provided. Described techniques allow deconvolving a first clonal population comprising a first plurality of cells of a species, wherein nucleic acid sequence reads from each cell in the first plurality of cells are obtained. The nucleic acid sequence reads are mapped into bins representing portions of a reference genome, and a pattern of sequence read counts for each cell across the multiple bins is used to assign a cell to a group, thereby inferring a mitotic status of the cell. The assignment of nucleic acid sequence reads into bins is also be used for segregating cells into classes based on a status of a certain biological marker in each cell. Comparison of sequence read counts for a subset of bins across the cell classes allows evaluating effect of a compound on a cell status.

公开(公告)号：US20230377688A1

公开(公告)日：2023-11-23

申请号：US18304718

申请日：2023-04-21

Applicant: 10X Genomics, Inc.

Inventor： Michael Schnall-Levin ,  Rajiv Bharadwaj ,  Zahra Kamila Belhocine ,  Andrew D. Price ,  Yifeng Yin ,  Vijay Kumar Sreenivasa Gopalan ,  Zeljko Jovan Dzakula

IPC: G16B30/00 ,  G16H10/40 ,  G16H20/00

CPC classification number: G16B30/00 ,  G16H10/40 ,  G16H20/00

Abstract: Systems and methods for inferring a status of a cell population are provided. Described techniques allow deconvolving a first clonal population comprising a first plurality of cells of a species, wherein nucleic acid sequence reads from each cell in the first plurality of cells are obtained. The nucleic acid sequence reads are mapped into bins representing portions of a reference genome, and a pattern of sequence read counts for each cell across the multiple bins is used to assign a cell to a group, thereby inferring a mitotic status of the cell. The assignment of nucleic acid sequence reads into bins is also be used for segregating cells into classes based on a status of a certain biological marker in each cell. Comparison of sequence read counts for a subset of bins across the cell classes allows evaluating effect of a compound on a cell status.