公开(公告)号：US20210324465A1

公开(公告)日：2021-10-21

申请号：US17232017

申请日：2021-04-15

Applicant: 10x Genomics, Inc.

Inventor： Preyas SHAH ,  Li WANG ,  Brett OLSEN

IPC: C12Q1/6874 ,  C12Q1/6806

Abstract: A method for conducting a customized analysis of open chromatin regions on a cell, comprising receiving an output file for a cell barcode genomic sequence dataset, the output file comprising a peak-barcode matrix, wherein each peak is defined by a plurality of fragment sequences aligned within a peak region, a unique barcode is associated with each fragment sequence in the dataset, and the peak-barcode matrix pairs each peak with the barcodes of the aligned fragments; and cell clusters with cells aggregated based on similarity in accessibility of specific transcription factor binding motifs associated with each cell, wherein the accessibility is determined via a differential accessibility analysis. The method further comprises adjusting customizable parameters for analyzing the peak-barcode matrix, and generating an updated output file including an updated clustering of cells, based on the customizable parameters, wherein each updated cell cluster includes cells with peaks representing a specific gene regulatory function.