公开(公告)号：US4434150A

公开(公告)日：1984-02-28

申请号：US313019

申请日：1981-10-19

申请人： A. R. M. Azad ,  Stefan J. Kirchanski ,  Michael C. Brown

发明人： A. R. M. Azad ,  Stefan J. Kirchanski ,  Michael C. Brown

IPC分类号： A61K45/08 ,  A61K39/395 ,  A61K47/48 ,  A61P35/00 ,  G01N33/53 ,  G01N33/531 ,  G01N33/532 ,  G01N33/543 ,  G01N33/58 ,  G01N33/54 ,  A61K43/00 ,  A61K49/00

CPC分类号： G01N33/532 ,  G01N33/58 ,  Y10S435/96 ,  Y10S435/962 ,  Y10S436/823

摘要： An immunological substance detecting reagent is provided which, in the preferred embodiment, employs an immunological homolog specific for the immunological substance to be detected coupled to a water-soluble polymer having a net charge not greater than zero. The water-soluble polymer further has associated a plurality of marker substances such as fluorophore molecules thereby providing a reagent of increased sensitivity for the detection of an antigen-antibody immunological reaction.

摘要翻译： 提供了一种免疫物质检测试剂，在优选实施方案中，其使用与要检测的免疫物质特异性的免疫同源物，其与净电荷不大于零的水溶性聚合物偶联。 水溶性聚合物还具有多个标记物质如荧光团分子，从而提供了检测抗原 - 抗体免疫反应的灵敏度增加的试剂。

公开(公告)号：US5053054A

公开(公告)日：1991-10-01

申请号：US407723

申请日：1989-09-14

申请人： Stefan J. Kirchanski ,  A. R. M. Azad ,  Peter J. Natale

发明人： Stefan J. Kirchanski ,  A. R. M. Azad ,  Peter J. Natale

IPC分类号： G01N1/30 ,  G01N33/52

CPC分类号： G01N1/30 ,  G01N33/52

摘要： Reagents and methods for intracellular staining of anionic materials as provided. The inherent staining characteristics of a fluorescent molecule may be suitably altered by covalently linking a plurality of said molecules to a nonimmunoglobulin derived cationic polymer having the desired staining specificity. By careful selection of the net charge and size of the dye polymer complex, diffusion through the cellular membrane can be enhanced and an enhanced fluorescent signal obtained by localization of a plurality of fluorescent molecules at the site of interest.

摘要翻译： 阴离子材料细胞内染色的试剂和方法。 荧光分子的固有染色特性可以通过将多个所述分子共价连接到具有所需染色特异性的非免疫球蛋白衍生的阳离子聚合物来适当地改变。 通过仔细选择染料聚合物复合物的净电荷和尺寸，可以增强通过细胞膜的扩散，并通过在感兴趣的位点定位多个荧光分子获得增强的荧光信号。

公开(公告)号：US5462867A

公开(公告)日：1995-10-31

申请号：US190732

申请日：1994-02-02

申请人： A. R. M. Azad ,  Randal A. Goffe

发明人： A. R. M. Azad ,  Randal A. Goffe

IPC分类号： C08F8/00 ,  C12N11/06 ,  C07C315/00 ,  C07K17/06 ,  G01N33/549

CPC分类号： C08F8/00 ,  Y10S436/824 ,  Y10S530/815 ,  Y10S530/816

摘要： A hydrophobic polymer such as polysulfone or polyethersulfone is modified to contain an increased number of functionalizable chain ends such as by treating with an alkali hydroxide to provide hydroxyl groups. A linker is covalently bonded to a chain end of the polymer and a macromolecule is covalently bonded to the linker. A ligand may be covalently bonded to the macromolecule. The macromolecule can be a natural polymer, a synthetic polymer or a biologically active species. The hydrophobic polymer is preferably in the form of a microporous membrane. By the use of a four-component dope composition, substantially isotropic microporous structures in the form of flat sheets or hollow fibers are produced. An improved spinnerette assembly is provided for the production of hollow fibers.

摘要翻译： 将疏水性聚合物如聚砜或聚醚砜改性为含有增加数量的可官能化链端，例如通过用碱金属氢氧化物处理以提供羟基。 接头共价键合到聚合物的链端，大分子与接头共价键合。 配体可以与大分子共价键合。 大分子可以是天然聚合物，合成聚合物或生物活性物质。 疏水性聚合物优选为微孔膜的形式。 通过使用四组分涂料组合物，产生基本上各向同性的平板或中空纤维形式的多孔结构。 提供改进的喷丝头组件用于生产中空纤维。