公开(公告)号：US10577620B2

公开(公告)日：2020-03-03

申请号：US16178702

申请日：2018-11-02

Applicant: ACADEMIA SINICA

Inventor： Hsou-Min Li ,  Chiung-Chih Chu

IPC: C12N15/82

Abstract: The present invention provides a recombinant DNA molecule encoding a fusion protein, comprising a first DNA sequence encoding a high-efficiency transit peptide operably linked to a second DNA sequence encoding a passenger protein, wherein the high-efficiency transit peptide is selected from the group consisting of transit peptides of the precursors of translocon at the inner envelope membrane of chloroplasts 40 kD (prTic40), chaperonin 10-2 (prCpn10-2), Fibrillin 1B (prFibrillin), ATP sulfurylase 1 (prAPS1), ATP sulfurylase 3 (prAPS3), 5′-adenylylsulfate reductase 3 (prAPR3), stromal ascorbate peroxidase (prsAPX), prTic40-E2A (a prTic40 variant), prCpn10-1-ΔC7C37S (a chaperonin 10-1 variant), a functional fragment of any of the transit peptides and an equivalent thereof. And the present invention also provides a method of high efficiency delivery of a protein into plastids using the high-efficiency transit peptides.

公开(公告)号：US20170016011A1

公开(公告)日：2017-01-19

申请号：US15210445

申请日：2016-07-14

Applicant: ACADEMIA SINICA

Inventor： Hsou-Min Li ,  Chiung-Chih Chu

IPC: C12N15/82

CPC classification number: C12N15/8214 ,  C12N15/8221

Abstract: The present invention provides a recombinant DNA molecule encoding a fusion protein, comprising a first DNA sequence encoding a high-efficiency transit peptide operably linked to a second DNA sequence encoding a passenger protein, wherein the high-efficiency transit peptide is selected from the group consisting of transit peptides of the precursors of translocon at the inner envelope membrane of chloroplasts 40 kD (prTic40), chaperonin 10-2 (prCpn10-2), Fibrillin 1B (prFibrillin), ATP sulfurylase 1 (prAPS1), ATP sulfurylase 3 (prAPS3), 5′-adenylylsulfate reductase 3 (prAPR3), stromal ascorbate peroxidase (prsAPX), prTic40-E2A (a prTic40 variant), prCpn10-1-ΔC7C37S (a chaperonin 10-1 variant), a functional fragment of any of the transit peptides and an equivalent thereof. And the present invention also provides a method of high efficiency delivery of a protein into plastids using the high-efficiency transit peptides.

Abstract translation: 本发明提供了编码融合蛋白的重组DNA分子，其包含编码与编码乘客蛋白质的第二DNA序列可操作地连接的高效转运肽的第一DNA序列，其中所述高效转运肽选自 叶绿体内包膜40kD（prTic40），伴侣蛋白10-2（prCpn10-2），纤维蛋白1A（prFibrillin），ATP硫化酶1（prAPS1），ATP硫化酶3（prAPS3））的转运蛋白前体的转运肽， ，5'-腺苷酸还原酶3（prAPR3），基质抗坏血酸过氧化物酶（prsAPX），prTic40-E2A（prTic40变体），prCpn10-1-ΔC7C37S（伴侣蛋白10-1变体），任何转运肽的功能片段 及其等同物。 并且本发明还提供了使用高效转运肽将蛋白质高效递送到质体中的方法。

公开(公告)号：US10577621B2

公开(公告)日：2020-03-03

申请号：US16179052

申请日：2018-11-02

Applicant: ACADEMIA SINICA

Inventor： Hsou-Min Li ,  Chiung-Chih Chu

IPC: C12N15/82

Abstract: The present invention provides a recombinant DNA molecule encoding a fusion protein, comprising a first DNA sequence encoding a high-efficiency transit peptide operably linked to a second DNA sequence encoding a passenger protein, wherein the high-efficiency transit peptide is selected from the group consisting of transit peptides of the precursors of translocon at the inner envelope membrane of chloroplasts 40 kD (prTic40), chaperonin 10-2 (prCpn10-2), Fibrillin 1B (prFibrillin), ATP sulfurylase 1 (prAPS1), ATP sulfurylase 3 (prAPS3), 5′-adenylylsulfate reductase 3 (prAPR3), stromal ascorbate peroxidase (prsAPX), prTic40-E2A (a prTic40 variant), prCpn10-1-ΔC7C37S (a chaperonin 10-1 variant), a functional fragment of any of the transit peptides and an equivalent thereof. And the present invention also provides a method of high efficiency delivery of a protein into plastids using the high-efficiency transit peptides.

公开(公告)号：US20190055573A1

公开(公告)日：2019-02-21

申请号：US16179052

申请日：2018-11-02

Applicant: ACADEMIA SINICA

Inventor： Hsou-Min Li ,  Chiung-Chih Chu

IPC: C12N15/82

Abstract: The present invention provides a recombinant DNA molecule encoding a fusion protein, comprising a first DNA sequence encoding a high-efficiency transit peptide operably linked to a second DNA sequence encoding a passenger protein, wherein the high-efficiency transit peptide is selected from the group consisting of transit peptides of the precursors of translocon at the inner envelope membrane of chloroplasts 40 kD (prTic40), chaperonin 10-2 (prCpn10-2), Fibrillin 1B (prFibrillin), ATP sulfurylase 1 (prAPS1), ATP sulfurylase 3 (prAPS3), 5′-adenylylsulfate reductase 3 (prAPR3), stromal ascorbate peroxidase (prsAPX), prTic40-E2A (a prTic40 variant), prCpn10-1-ΔC7C37S (a chaperonin 10-1 variant), a functional fragment of any of the transit peptides and an equivalent thereof. And the present invention also provides a method of high efficiency delivery of a protein into plastids using the high-efficiency transit peptides.

公开(公告)号：US20190055572A1

公开(公告)日：2019-02-21

申请号：US16178702

申请日：2018-11-02

Applicant: ACADEMIA SINICA

Inventor： Hsou-Min Li ,  Chiung-Chih Chu

IPC: C12N15/82

Abstract: The present invention provides a recombinant DNA molecule encoding a fusion protein, comprising a first DNA sequence encoding a high-efficiency transit peptide operably linked to a second DNA sequence encoding a passenger protein, wherein the high-efficiency transit peptide is selected from the group consisting of transit peptides of the precursors of translocon at the inner envelope membrane of chloroplasts 40 kD (prTic40), chaperonin 10-2 (prCpn10-2), Fibrillin 1B (prFibrillin), ATP sulfurylase 1 (prAPS1), ATP sulfurylase 3 (prAPS3), 5′-adenylylsulfate reductase 3 (prAPR3), stromal ascorbate peroxidase (prsAPX), prTic40-E2A (a prTic40 variant), prCpn10-1-ΔC7C37S (a chaperonin 10-1 variant), a functional fragment of any of the transit peptides and an equivalent thereof. And the present invention also provides a method of high efficiency delivery of a protein into plastids using the high-efficiency transit peptides.

公开(公告)号：US10184128B2

公开(公告)日：2019-01-22

申请号：US15210445

申请日：2016-07-14

Applicant: ACADEMIA SINICA

Inventor： Hsou-Min Li ,  Chiung-Chih Chu

IPC: C12N15/82

Abstract: The present invention provides a recombinant DNA molecule encoding a fusion protein, comprising a first DNA sequence encoding a high-efficiency transit peptide operably linked to a second DNA sequence encoding a passenger protein, wherein the high-efficiency transit peptide is selected from the group consisting of transit peptides of the precursors of translocon at the inner envelope membrane of chloroplasts 40 kD (prTic40), chaperonin 10-2 (prCpn10-2), Fibrillin 1B (prFibrillin), ATP sulfurylase 1 (prAPS1), ATP sulfurylase 3 (prAPS3), 5′-adenylylsulfate reductase 3 (prAPR3), stromal ascorbate peroxidase (prsAPX), prTic40-E2A (a prTic40 variant), prCpn10-1-ΔC7C37S (a chaperonin 10-1 variant), a functional fragment of any of the transit peptides and an equivalent thereof. And the present invention also provides a method of high efficiency delivery of a protein into plastids using the high-efficiency transit peptides.