公开(公告)号：US20190269227A1

公开(公告)日：2019-09-05

申请号：US16415411

申请日：2019-05-17

Applicant: ACADEMIA SINICA

Inventor： Chi-Huey WONG ,  Tsui-Ling HSU ,  Sarah R. HANSON ,  Masaaki SAWA

IPC: A45F3/14 ,  A41F9/02 ,  A45F5/02 ,  A45F3/00 ,  A45F3/10

Abstract: The present disclosure relates to a method for metabolic oligosaccharide engineering that incorporates derivatized alkyne-bearing sugar analogs as “tags” into cellular glycoconjugates. The disclosed method incorporates alkynyl derivatized Fuc and alkynyl derivatized ManNAc sugars into a cellular glycoconjugate. A chemical probe comprising an azide group and a visual probe or a fluorogenic probe is used to label the alkyne-derivatized sugar-tagged glycoconjugate. In one aspect, the chemical probe binds covalently to the alkynyl group by Cu(I)-catalyzed [3+2] azide-alkyne cycloaddition and is visualized at the cell surface, intracellularly, or in a cellular extract. The labeled glycoconjugate is capable of detection by flow cytometry, SDS-PAGE, Western blot, ELISA or confocal microscopy, and mass spectrometry.