公开(公告)号：US20160068901A1

公开(公告)日：2016-03-10

申请号：US14888137

申请日：2014-05-01

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Allen E. Eckhardt ,  Michael G. Pollack ,  David S. Cohen

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  B01L7/52 ,  C12Q1/6844 ,  C40B20/00 ,  C12Q2533/101 ,  C12Q2565/301

Abstract: The invention provides pyrosequencing-based methods of analyzing and synthesizing DNA, including methods of DNA error correction, determining DNA size distribution, screening for nucleotide repeat disorders such as fragile X syndrome, determining size distribution and bias in a DNA library, and determining pyrosequencing read length. The methods include on-bench protocols as well as droplet-based protocols that may be conducted on a droplet actuator.

Abstract translation: 本发明提供了基于焦磷酸测序的DNA分析和合成方法，包括DNA错误校正方法，确定DNA大小分布，筛选核苷酸重复序列如脆性X综合征，确定DNA文库中的大小分布和偏差，以及确定焦磷酸测序读数 长度。 这些方法包括工作台协议以及可以在液滴致动器上进行的基于液滴的协议。