公开(公告)号：US11378558B2

公开(公告)日：2022-07-05

申请号：US16772828

申请日：2019-02-21

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Sern Farh Matthew Choo ,  Terry Nguyen-Khuong ,  Pauline Mary Rudd

IPC: G01N30/72 ,  G01N30/86 ,  G01N30/88

Abstract: A method identifies glycopeptides in a sample. The method includes converting a mass spectrum of MS1 precursors of the sample into a plurality of nodes in a graph, each node corresponding to one mass and one retention time of a glycopeptide to be identified in the sample; calculating differences in the mass and/or retention time between all combinations of pairs of the nodes; generating a graph theoretic network of the nodes; and predicting compositions of the glycopeptides in the sample based on the graph theoretic network of the nodes so as to identify the glycopeptides.

公开(公告)号：US20210164947A1

公开(公告)日：2021-06-03

申请号：US16772828

申请日：2019-02-21

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Sern Farh Matthew Choo ,  Terry Nguyen-Khuong ,  Pauline Mary Rudd

IPC: G01N30/72 ,  G01N30/86 ,  G01N30/88

Abstract: A method identifies glycopeptides in a sample. The method includes converting a mass spectrum of MS1 precursors of the sample into a plurality of nodes in a graph, each node corresponding to one mass and one retention time of a glycopeptide to be identified in the sample; calculating differences in the mass and/or retention time between all combinations of pairs of the nodes; generating a graph theoretic network of the nodes; and predicting compositions of the glycopeptides in the sample based on the graph theoretic network of the nodes so as to identify the glycopeptides.

公开(公告)号：US20220251619A1

公开(公告)日：2022-08-11

申请号：US17598965

申请日：2020-03-23

Applicant: Agency For Science, Technology And Research ,  The University of Manchester

Inventor： Sern Farh Matthew Choo ,  Terry Nguyen-Khuong ,  Edward George Pallister ,  Say Kong Ng ,  Sabine Lahja Flitsch

IPC: C12P21/00 ,  C07K1/107

Abstract: The invention relates to a method of increasing the number of α2,3,-α2,6-disialylgalactose N-glycans on a glycoprotein by incubating an α2,3-sialylated glycoprotein with an α2,6-sialyltransferase and a sialic acid source. Also provided is a recombinant glycoprotein comprising at least one α2,3,-α2,6-disialylgalactose N-glycan. In a particular embodiment, the recombinant glycoprotein is alpha-1 antitrypsin (AAT).