公开(公告)号：US20230079226A1

公开(公告)日：2023-03-16

申请号：US17797950

申请日：2021-02-10

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Jiaxu WANG ,  Yue WAN

IPC: C12Q1/6806 ,  C12Q1/6869

Abstract: The invention relates to a method of determining a structure of ribonucleic acid (RNA) molecules, the method comprising: contacting the RNA molecules with a modifying agent to obtain modified RNA molecules; reverse transcribing the modified RNA molecules; sequencing the product obtained from the preceding step to generate sequencing reads; and analysing the sequencing reads to determine the structure of the RNA molecules. In a preferred embodiment, the RNA molecules consist of RNA molecules of a single cell, and the modifying agent is 2-methylnicotinic acid imidazolide-azide (NAI-N3). There is also provided a method of classifying cells into one or more cell populations, the method comprising: determining the structure of RNA molecules in each cell; and classifying the cells into one or more cell populations based on similarity in the structure of their RNA molecules.