公开(公告)号：US11525132B2

公开(公告)日：2022-12-13

申请号：US17210094

申请日：2021-03-23

Applicant: AJINOMOTO CO., INC.

Inventor： Miwa Konishi ,  Shohei Kajimoto ,  Yusuke Hagiwara ,  Yasuhiro Mihara ,  Shogo Nakano ,  Tomoharu Motoyama ,  Sohei Ito

IPC: C12N9/00 ,  C12N15/11 ,  C12P19/34

Abstract: Ligase mutants of the following (1), (2), or (3):
(1) a ligase mutant comprising an amino acid sequence showing 95% or more identity to the amino acid sequence of SEQ ID NO: 1, and having a nucleic acid-linking activity;
(2) a ligase mutant comprising an amino acid sequence showing 90% or more identity to the amino acid sequence of SEQ ID NO: 2, and having a nucleic acid-linking activity; or
(3) a ligase mutant comprising an amino acid sequence showing 97% or more identity to the amino acid sequence of SEQ ID NO: 3, and having a nucleic acid-linking activity, have excellent properties.