公开(公告)号：US20160222055A1

公开(公告)日：2016-08-04

申请号：US15135012

申请日：2016-04-21

Applicant: AJINOMOTO CO., INC.

Inventor： Takahiro NONAKA ,  Teruhisa MANNEN ,  Noriko TSURUI

IPC: C07K1/30 ,  C07K1/36 ,  C07K14/34 ,  C07K14/62 ,  C07K14/635 ,  C07K1/34 ,  C07K14/815

CPC classification number: C07K1/30 ,  C07K1/34 ,  C07K1/36 ,  C07K14/00 ,  C07K14/32 ,  C07K14/34 ,  C07K14/62 ,  C07K14/635 ,  C07K14/815 ,  C07K2319/00 ,  C07K2319/50 ,  C07K2319/70

Abstract: The present invention provides a method for producing a target protein in the form of a fusion protein at a high recovery ratio. The present invention relates to a method for producing a fusion protein made of a protein having a self-assembly capability and a target protein comprising the following steps (1) to (4):(1) preparing a solution containing the fusion protein;(2) adjusting a pH of the solution obtained in step (1) to such a pH that a recovery ratio calculated according to the following equation is 10% or more, where the recovery ratio (%)=[an amount of the fusion protein in a solution obtained in step (4)/{the amount of the fusion protein in the solution obtained in step (4)+an amount of the fusion protein in a solution after solid separation in step (3)}]×100; (3) separating a solid from the solution obtained in step (2); and(4) dissolving the solid separated in step (3) into a solution having a pH of 12 or below but higher than the pH of the solution obtained in step (2) by 0.1 or more.