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公开(公告)号：US10023608B1
公开(公告)日：2018-07-17
申请号：US14208043
申请日：2014-03-13
Applicant: AMGEN INC.
Inventor： Junfen Ma , Xiaoyang Zhao , Brian Williamson
Abstract: Disclosed are methods of purifying a protein sample. More specifically, provide are methods of removing or reducing the amount of high molecular weight species and/or high mannose species from a protein sample using a mixed mode chromatography step and a hydrophobic interaction chromatography step.
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