公开(公告)号：US11299760B2

公开(公告)日：2022-04-12

申请号：US17464115

申请日：2021-09-01

Applicant: Amgen Inc.

Inventor： Sandhya Pande ,  Mirna Mujacic

IPC: C12P21/00

Abstract: Methods of modulating the properties of a cell culture expressing a protein of interest are provided. In various embodiments the methods relate to the addition of cell-cycle inhibitors to growing cell cultures.

公开(公告)号：US11130980B2

公开(公告)日：2021-09-28

申请号：US15033559

申请日：2014-10-30

Applicant: AMGEN INC.

Inventor： Sandhya Pande ,  Mirna Mujacic

IPC: C12N5/02 ,  C12P21/00

Abstract: Methods of modulating high mannose glycoform content of a protein in a cell culture by contacting the cells expressing the protein of interest with monesin are provided.