公开(公告)号：US20220260584A1

公开(公告)日：2022-08-18

申请号：US17616366

申请日：2020-06-05

Applicant: AMGEN INC.

Inventor： Pavel BONDARENKO ,  Scott Thomas KUHNS ,  Andrew NICHOLS ,  Gang XIAO ,  Liuqing SHI ,  Jill A. CROUSE-ZEINEDDINI

IPC: G01N33/68 ,  G01N33/543 ,  G01N33/547

Abstract: Provided herein are methods of identifying structures, e.g., attributes, of a therapeutic protein or a target that affect an interaction between the therapeutic protein and the target. In exemplary embodiments, the method comprises: (a) applying a stress to a first sample comprising therapeutic proteins or targets; (b) contacting the first sample with a second sample comprising targets or therapeutic proteins to form a mixture comprising (i) therapeutic protein-target complexes, (ii) unbound therapeutic proteins, and (iii) unbound targets; and (c) separating the mixture into at least two fractions, wherein an unbound fraction comprises unbound therapeutic proteins or unbound targets and a bound fraction comprises therapeutic protein-target complexes; and (d) for each of the unbound fraction and bound fraction, identifying and quantifying the abundance of the structures, e.g., attribute, present on a species of the therapeutic protein or target.