公开(公告)号：US20110114488A1

公开(公告)日：2011-05-19

申请号：US13013069

申请日：2011-01-25

申请人： ANDERS LARSSON ,  RONNIE PALMGREN ,  SOFIA SÖDERBERG

发明人： ANDERS LARSSON ,  RONNIE PALMGREN ,  SOFIA SÖDERBERG

IPC分类号： G01N27/447

CPC分类号： G01N27/44747

摘要： The present invention relates to electrophoresis and in particular low fluorescent electrophoretic supports for hydrogels used for separation of fluorescence labelled biomolecules. More particularly, the invention relates to use of a polymer having the following formula: wherein n=0-100 000 m=0-100 000 R1, R2, R3 and R4=H, F, Cl, Br, I, methyl groups or non-aromatic hydrocarbon chains (optionally containing branches or cyclic structures) such as ethyl, ethenyl, propyl, isopropyl, propenyl, butyl, branched butyl, butenyl, cyclobutyl, pentyl, branched pentyl, pentenyl, cyclopentyl, hexyl, branched hexyl, cyclohexyl; X, Y=methylene groups or non-aromatic hydrocarbon chains (optionally containing branches or cyclic structures) such as ethylene, ethenylene, propylene, isopropylene, propenylene, butylene, branched butylene, butenylene; Y can optionally be absent as a low fluorescent support film of an electrophoretic hydrogel for slab gel electrophoresis. The invention also relates to composites of such films and hydrogels as well as kits for 2D electrophoresis.

摘要翻译： 本发明涉及电泳，特别是用于分离荧光标记的生物分子的水凝胶的低荧光电泳载体。 更具体地说，本发明涉及具有下式的聚合物的用途：其中n = 0-100000m = 0-100000 R1，R2，R3和R4 = H，F，Cl，Br，I，甲基或 乙烯基，乙烯基，丙基，异丙基，丙烯基，丁基，支链丁基，丁烯基，环丁基，戊基，支链戊基，戊烯基，环戊基，己基，支链己基，环己基等非芳族烃链（任选含有支链或环状结构） X，Y =亚甲基或非芳族烃链（任选地含有支链或环状结构）如乙烯，亚乙烯基，丙烯，异丙烯，亚丙烯基，亚丁基，支链亚丁基，亚丁烯基; 作为板状凝胶电泳用电泳水凝胶的低荧光支持膜，Y可任选地不存在。 本发明还涉及这种膜和水凝胶的复合材料以及用于2D电泳的试剂盒。