公开(公告)号：US08847004B2

公开(公告)日：2014-09-30

申请号：US13372787

申请日：2012-02-14

申请人： Andrew J. Murphy ,  Sean Stevens ,  Chozhavendan Rathinam ,  Elizabeth Eynon ,  Markus Manz ,  Richard Flavell ,  George D. Yancopoulos

发明人： Andrew J. Murphy ,  Sean Stevens ,  Chozhavendan Rathinam ,  Elizabeth Eynon ,  Markus Manz ,  Richard Flavell ,  George D. Yancopoulos

IPC分类号： A01K67/027

CPC分类号： A01K67/0278 ,  A01K67/027 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2207/15 ,  A01K2217/072 ,  A01K2217/15 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0337 ,  A61K49/0008 ,  C12N15/8509 ,  C12N2015/8536 ,  G01N33/5088 ,  G01N2500/10

摘要： Genetically modified mice comprising a nucleic acid sequence encoding a human M-CSF protein are provided. Also provided are genetically modified mice comprising a nucleic acid sequence encoding a human M-CSF protein that have been engrafted with human cells such as human hematopoietic cells, and methods for making such engrafted mice. These mice find use in a number of applications, such as in modeling human immune disease and pathogen infection; in in vivo screens for agents that modulate hematopoietic cell development and/or activity, e.g. in a healthy or a diseased state; in in vivo screens for agents that are toxic to hematopoietic cells; in in vivo screens for agents that prevent against, mitigate, or reverse the toxic effects of toxic agents on hematopoietic cells; in in vivo screens of human hematopoietic cells from an individual to predict the responsiveness of an individual to a disease therapy, etc.

摘要翻译： 提供了包含编码人M-CSF蛋白的核酸序列的转基因小鼠。 还提供了包含编码人类细胞如人类造血细胞的人M-CSF蛋白质的核酸序列的遗传修饰的小鼠，以及用于制备这种移植的小鼠的方法。 这些小鼠可用于许多应用，例如建模人类免疫疾病和病原体感染; 在用于调节造血细胞发育和/或活性的试剂的体内筛选中。 处于健康或疾病状态; 在对造血细胞有毒性的药物的体内筛选中; 在体内筛选用于防止，减轻或逆转毒性剂对造血细胞的毒性作用的药剂; 在来自个体的人类造血细胞的体内筛选中以预测个体对疾病治疗的反应性等。

公开(公告)号：US20130042330A1

公开(公告)日：2013-02-14

申请号：US13372787

申请日：2012-02-14

申请人： ANDREW J. MURPHY ,  Sean Stevens ,  Chozhavendan Rathinam ,  Elizabeth Eynon ,  Markus Manz ,  Richard Flavell

发明人： ANDREW J. MURPHY ,  Sean Stevens ,  Chozhavendan Rathinam ,  Elizabeth Eynon ,  Markus Manz ,  Richard Flavell

IPC分类号： A01K67/027

CPC分类号： A01K67/0278 ,  A01K67/027 ,  A01K67/0271 ,  A01K2207/12 ,  A01K2207/15 ,  A01K2217/072 ,  A01K2217/15 ,  A01K2227/105 ,  A01K2267/03 ,  A01K2267/0337 ,  A61K49/0008 ,  C12N15/8509 ,  C12N2015/8536 ,  G01N33/5088 ,  G01N2500/10

摘要： Genetically modified mice comprising a nucleic acid sequence encoding a human M-CSF protein are provided. Also provided are genetically modified mice comprising a nucleic acid sequence encoding a human M-CSF protein that have been engrafted with human cells such as human hematopoietic cells, and methods for making such engrafted mice. These mice find use in a number of applications, such as in modeling human immune disease and pathogen infection; in in vivo screens for agents that modulate hematopoietic cell development and/or activity, e.g. in a healthy or a diseased state; in in vivo screens for agents that are toxic to hematopoietic cells; in in vivo screens for agents that prevent against, mitigate, or reverse the toxic effects of toxic agents on hematopoietic cells; in in vivo screens of human hematopoietic cells from an individual to predict the responsiveness of an individual to a disease therapy, etc.

摘要翻译： 提供了包含编码人M-CSF蛋白的核酸序列的转基因小鼠。 还提供了包含编码人类细胞如人类造血细胞的人M-CSF蛋白质的核酸序列的遗传修饰的小鼠，以及用于制备这种移植的小鼠的方法。 这些小鼠可用于许多应用，例如建模人类免疫疾病和病原体感染; 在用于调节造血细胞发育和/或活性的试剂的体内筛选中。 处于健康或疾病状态; 在对造血细胞有毒性的药物的体内筛选中; 在体内筛选用于防止，减轻或逆转毒性剂对造血细胞的毒性作用的药剂; 在来自个体的人类造血细胞的体内筛选中以预测个体对疾病治疗的反应性等。