公开(公告)号：US20240294674A1

公开(公告)日：2024-09-05

申请号：US18254495

申请日：2021-11-25

申请人： ASSEMBLY MEDICINE, LLC.

发明人： Fan Yang ,  James Jeiwen Chou ,  Liujuan Zhou ,  Yanbing Wang ,  Chan Cao ,  Changqing Run

IPC分类号： C07K19/00 ,  A61K47/54 ,  C07K14/535 ,  G16B15/10

CPC分类号： C07K19/00 ,  A61K47/549 ,  C07K14/535 ,  G16B15/10 ,  C07K2317/569

摘要： A construction method for and an application of a nucleic acid multimerization-mediated multivalent protein drug and vaccine. Specifically provided is a multimeric complex based on complementary nucleic acid backbones. The complex is a multimer formed by complexing of 3-6 monomers having complementary nucleic acid backbones, wherein each monomer is a polypeptide having a nucleic acid single strand. In the multimer, the nucleic acid single strand of each monomer and the nucleic acid single strands of the other two monomers form double strands by means of base complementation, so as to form complementary nucleic acid backbone structures. Also provided are a pharmaceutical composition containing the multimeric complex, a nucleic acid sequence library used for constructing the multimeric complex, and a method for optimizing complementary nucleic acid backbones. By means of the method, off-the-shelf short-acting protein drugs or antigens can be used to complete multivalent formation of protein drugs or antigens without the need of reconstruction of fusion proteins or chemical modification and cross-linking, thereby improving their half-life and activity, and/or immunogenicity.