公开(公告)号：US20220018833A1

公开(公告)日：2022-01-20

申请号：US17488095

申请日：2021-09-28

Applicant: Access Medical Systems, Ltd.

Inventor： Robert F. Zuk ,  Qing Xia ,  Jenchieh Wu

IPC: G01N33/543 ,  G01N21/64

Abstract: The present invention is directed immunoassay methods, which re-use a hapten-immobilized test probe and reagents for quantitating an analyte in different samples, anywhere from about 3 to 20 times, while maintaining acceptable clinical assay performance. The methods use a dual antibody conjugate solution comprising an anti-hapten antibody and a capture antibody against the analyte in each cycle. After the completion of each cycle of reaction, the test probe is dipped in an acidic solution having pH about 1-4, to elute the immunocomplex formed on the probe and to regenerate the hapten-immobilized probe. The robustness of the hapten-coated solid phase allows utilization of multiple denaturation reagents for efficient elution of the immune complexes after each cycle without compromising the binding activity of the hapten on the solid phase.