公开(公告)号：US20240168011A1

公开(公告)日：2024-05-23

申请号：US18549388

申请日：2022-03-08

申请人： Akershus Universitetssykehus HF

发明人： Tormod FLADBY ,  Marianne WETTERGREEN ,  Kulbhushan SHARMA ,  Berglind GISLADOTTIR ,  Bjørn-Eivind KIRSEBOM

IPC分类号： G01N33/50

CPC分类号： G01N33/5035 ,  G01N33/5055 ,  G01N2333/4709 ,  G01N2800/2821

摘要： An ex vivo method of determining the effect of an agent on catalysis by a Monocyte Phagocyte System (MPS) cell of a disease marker product and/or at least one fragment thereof associated with neurodegeneration and/or inflammatory activation. The method comprises: i) maintaining a sample of MPS cells under conditions in which the MPS cells remain alive, ii) exposing the sample of MPS cells to an agent and a disease marker product, to permit phagocytosis of the disease marker product by the MPS cells, iii) detecting the intracellular amount of the disease marker product and/or at least one fragment thereof in the sample of MPS cells, and iv) comparing the intracellular amount of the disease marker product and/or the at least one fragment thereof to an intracellular amount of the same disease marker product and/or at least one fragment thereof measured in control MPS cells in the absence of the agent. The effect of the agent on catalysis by MPS cells of the disease marker product and/or at least one fragment thereof is determined by the result of the comparison of step iv).