公开(公告)号：US06828416B1

公开(公告)日：2004-12-07

申请号：US09763397

申请日：2001-02-16

申请人： Altaf A. Lal ,  Ya Ping Shi ,  Seyed E. Hasnain

发明人： Altaf A. Lal ,  Ya Ping Shi ,  Seyed E. Hasnain

IPC分类号： C07K700

CPC分类号： C07K14/445 ,  A61K39/00 ,  C07K2319/02 ,  Y02A50/412

摘要： A recombinant protein is provided which comprises peptides derived from different stages in the life cycle of parasite Plasmodium falciparum. The protein is useful as a reagent and, when combined with a pharmaceutically-acceptable vehicle or carrier, is useful as a vaccine against the material parasite Plasmodium falciparum. A genetic construct used to produce this recombinant protein vaccine is also described. In addition, antibodies to this recombinant protein are provided which are useful for the detection and measurement of peptides derived from different stages in the life cycle of the parasite Plasmodium falciparum.

摘要翻译： 提供了重组蛋白，其包含衍生自寄生虫恶性疟原虫生命周期中不同阶段的肽。 该蛋白质可用作试剂，并且当与药学上可接受的载体或载体组合时，可用作针对物质寄生虫恶性疟原虫的疫苗。 还描述了用于产生该重组蛋白疫苗的遗传构建体。 此外，提供了对该重组蛋白的抗体，其可用于检测和测量衍生自寄生虫恶性疟原虫生命周期中的不同阶段的肽。

公开(公告)号：US5965393A

公开(公告)日：1999-10-12

申请号：US886595

申请日：1997-07-01

申请人： Seyed E. Hasnain ,  Betapudi Venkaiah ,  Saman Habib

发明人： Seyed E. Hasnain ,  Betapudi Venkaiah ,  Saman Habib

IPC分类号： C12N15/866 ,  C12N15/86 ,  C12N5/10 ,  C12P21/00

CPC分类号： C12N15/86 ,  C12N2710/14143

摘要： The baculovirus homologous region (hr1) sequence has been disclosed as a transcriptional enhancer of foreign gene expression. An additional copy of the hr1 element contained within a recombinant baculovirus has been demonstrated to be capable of further enhancing expression of foreign genes placed under the transcriptional control of a promoter such as the baculovirus polyhedrin and/or Drosophila hsp70 promoter. The recombinant baculovirus is stable for up to 30 passages. This invention can be employed to generate recombinant expression vector plasmids and recombinant baculoviruses that can be used to produce increased levels of recombinant protein products of therapeutic, biomedical or basic science research interest.

摘要翻译： 已经公开了杆状病毒同源区（hr1）序列作为外源基因表达的转录增强子。 重组杆状病毒中包含的hr1元件的额外拷贝已被证明能够进一步增强置于启动子如杆状病毒多角体蛋白和/或果蝇hsp70启动子的转录控制下的外源基因的表达。 重组杆状病毒对于多达30代是稳定的。 本发明可用于产生重组表达载体质粒和重组杆状病毒，其可用于产生增加水平的治疗，生物医学或基础科学研究兴趣的重组蛋白产物。