公开(公告)号：US20220326498A1

公开(公告)日：2022-10-13

申请号：US17718632

申请日：2022-04-12

Applicant: Andor Technology Limited

Inventor： John Oreopoulos ,  Peter Sinclair

IPC: G02B21/08 ,  G02B21/02 ,  F21V8/00

Abstract: A microscope system has an illumination optical system comprising a multi-mode optical fibre having an egress for emitting a laser beam. The egress is located in a plane that is conjugate to the microscope sample plane. The illumination optical system is configured such that the laser beam is incident at the objective lens laterally displaced from the principal optical axis of the objective lens in order that the objective lens delivers the laser beam to the sample plane at an angle that is oblique to the principal optical axis. Utilization of a multi-mode optical fibre for laser delivery in oblique illumination microscopy, such as TIRF microscopy, solves problems associated with using single-mode optical fibres such as alignment and uniformity of illumination.