公开(公告)号：US20090247898A1

公开(公告)日：2009-10-01

申请号：US12392045

申请日：2009-02-24

申请人： Andrea ROBITZKI ,  Andree Rothermel ,  Heinz-Georg Jahnke ,  Ina Sternberger ,  Frank Striggow ,  Till Mack

发明人： Andrea ROBITZKI ,  Andree Rothermel ,  Heinz-Georg Jahnke ,  Ina Sternberger ,  Frank Striggow ,  Till Mack

IPC分类号： A61B5/053

CPC分类号： G01N33/4836 ,  G01N27/041

摘要： The present invention refers to a device for measuring impedance in organotypic tissue comprising at least one recording chamber with a liquid permeable membrane supporting the organotypic tissue, at least one bottom electrode and at least one top electrode, wherein the liquid permeable membrane divides the recording chamber into a top chamber and a bottom chamber, wherein at least the bottom chamber contains culture medium for the organotypic tissue, and the bottom electrode(s) is/are located in the bottom chamber and the top electrode(s) is/are located in the top chamber, and wherein the organotypic tissue is located between the bottom electrode(s) and the top electrode(s). The present invention also refers to the use of the device according to the present invention for measuring impedance in organotypic tissue.Furthermore, the present invention relates to a method for analyzing the effect of test compounds on pathological and non-pathological organotypic tissue by measuring the impedance of the organotypic tissue, wherein the organotypic tissue is cultured in a culture medium during the time of the analysis and the impedance of the organotypic tissue is measured at least once before and at least once after treating the organotypic tissue with the test compound or the impedance of the organotypic tissue treated with the test compound is compared to a non-treated organotypic tissue, wherein the impedance is measured using at least one electrode at each of two opposing sides of the organotypic tissue, and the electrodes are contacted with the culture medium or the tissue during measuring the impedance.

摘要翻译： 本发明涉及一种用于测量器官组织中的阻抗的装置，包括至少一个具有支撑器官组织的液体可渗透膜的记录室，至少一个底部电极和至少一个顶部电极，其中液体可透膜将记录室 进入顶部腔室和底部腔室，其中至少底部腔室包含用于器官型组织的培养基，并且底部电极位于底部腔室中，并且顶部电极位于 顶部腔室，并且其中所述器官型组织位于所述底部电极和所述顶部电极之间。 本发明还涉及根据本发明的装置用于测量器官组织中的阻抗的用途。 此外，本发明涉及通过测量器官型组织的阻抗来分析试验化合物对病理和非病理性器官组织的影响的方法，其中在分析期间将培养基培养在器官型组织中， 在用测试化合物处理器官型组织之后至少一次测量器官型组织的阻抗，或者将用受试化合物治疗的器官型组织的阻抗与未处理的器官类型组织进行比较，其中阻抗 使用在器官型组织的两个相对侧中的每一侧上的至少一个电极测量，并且在测量阻抗期间电极与培养基或组织接触。