公开(公告)号：US20210079337A1

公开(公告)日：2021-03-18

申请号：US17040427

申请日：2019-03-29

申请人： Arizona Board of Regents on Behalf of the University of Arizona

发明人： Frederic ZENHAUSERN ,  Matthew W. BARRETT ,  Carla BROOKS ,  Peng CHEN ,  Brett M. DUANE ,  Marie Oceane PARENT ,  Stanley D. SMITH ,  Baiju THOMAS ,  Jianing YANG

IPC分类号： C12M1/36 ,  C12M3/06 ,  C12M1/00 ,  C12M1/12 ,  C12M3/00

摘要： Provided are automated cell culture systems and related in vitro cell culture methods, including for a co-culture of different cell populations having different cell culture conditions. For example, the system may comprise a bioreactor having a plurality of cell culture compartments, the bioreactor having a fluid port fluidly connected to the plurality of cell culture compartments. A pump fluidly connected to the fluid port can provide a cell culture medium to the plurality of culture compartments. A sensor is operably connected to at least one of the plurality of cell culture compartments for measuring at least one cell culture parameter. In this manner, a controller electronically connected the pump and sensor, is configured to automatically adjust a pump flow rate and/or a cell culture gas content to provide a desired steady-state cell culture parameter for facilitating cells interactions and collection of products representative of said interactions.