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1.发明授权Oligonucleotides, use thereof, detecting method and kit for diagnosing the presence of H5 and N1 genes of the Influenza A virus 有权寡核苷酸，其用途，用于诊断甲型流感病毒H5和N1基因存在的检测方法和试剂盒公开(公告)号：US08168387B2
公开(公告)日：2012-05-01
申请号：US12084704
申请日：2006-11-23
申请人： Aurélie Lefeuvre , Jean-Noel Telles , Guy Vernet
发明人： Aurélie Lefeuvre , Jean-Noel Telles , Guy Vernet
CPC分类号： C12Q1/701
摘要： The present invention relates to a double pair of oligonucleotides for amplifying two target sequences located, respectively, in the H5 and N1 genes of the genome of the Influenza A virus, said oligonucleotides being of a length ranging between 10 and 50 nucleotides and comprising at least one fragment of 10 consecutive nucleotides derived from the following sequences: SEQ ID No. 1:TGTATGTTGTGGAATGGCA, SEQ ID No. 2:GCCGAATGATGCCATCAA, SEQ ID No. 3:CGTGGATTGTCTCCGAAA, and SEQ ID No. 4:GGAATGCTCCTGTTATCCTGA or the sequence complementary thereto. The invention also relates to oligonucleotides for detecting amplicons, to the use of all these sequences, and to a method for detecting and a kit for diagnosing the presence of the H5 and N1 genes of the Influenza A virus.The invention is particularly applicable in the field of diagnosis.
摘要翻译： 本发明涉及用于扩增分别位于甲型流感病毒基因组的H5和N1基因中的两个靶序列的双重寡核苷酸，所述寡核苷酸的长度范围在10至50个核苷酸之间并且至少包含 衍生自以下序列的10个连续核苷酸的一个片段：SEQ ID No.1：TGTATGTTGTGGAATGGCA，SEQ ID No.2：GCCGAATGATGCCATCAA，SEQ ID No.3：CGTGGATTGTCTCCGAAA和SEQ ID No.4：GGAATGCTCCTGTTATCCTGA或其互补序列。 本发明还涉及用于检测扩增子的寡核苷酸，所有这些序列的使用以及用于检测的方法和用于诊断甲型流感病毒的H5和N1基因的存在的试剂盒。 本发明特别适用于诊断领域。
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2.发明申请Oligonucleotides, Use Thereof, Detecting Method and Kit for Diagnosing the Presence of H5 and N1 Genes of the Influenza a Virus 有权寡核苷酸，其用途，用于诊断流感病毒H5和N1基因存在的检测方法和试剂盒公开(公告)号：US20090305243A1
公开(公告)日：2009-12-10
申请号：US12084704
申请日：2006-11-23
申请人： Aurélie Lefeuvre , Jean-Noel Telles , Guy Vernet
发明人： Aurélie Lefeuvre , Jean-Noel Telles , Guy Vernet
CPC分类号： C12Q1/701
摘要： The present invention relates to a double pair of oligonucleotides for amplifying two target sequences located, respectively, in the H5 and N1 genes of the genome of the Influenza A virus, said oligonucleotides being of a length ranging between 10 and 50 nucleotides and comprising at least one fragment of 10 consecutive nucleotides derived from the following sequences: SEQ ID No. 1:TGTATGTTGTGGAATGGCA, SEQ ID No. 2:GCCGAATGATGCCATCAA, SEQ ID No. 3:CGTGGATTGTCTCCGAAA, and SEQ ID No. 4:GGAATGCTCCTGTTATCCTGA or the sequence complementary thereto. The invention also relates to oligonucleotides for detecting amplicons, to the use of all these sequences, and to a method for detecting and a kit for diagnosing the presence of the H5 and N1 genes of the Influenza A virus.The invention is particularly applicable in the field of diagnosis.
摘要翻译： 本发明涉及用于扩增分别位于甲型流感病毒基因组的H5和N1基因中的两个靶序列的双重寡核苷酸，所述寡核苷酸的长度范围在10至50个核苷酸之间并且至少包含 衍生自以下序列的10个连续核苷酸的一个片段：SEQ ID No.1：TGTATGTTGTGGAATGGCA，SEQ ID No.2：GCCGAATGATGCCATCAA，SEQ ID No.3：CGTGGATTGTCTCCGAAA和SEQ ID No.4：GGAATGCTCCTGTTATCCTGA或其互补序列。 本发明还涉及用于检测扩增子的寡核苷酸，所有这些序列的使用以及用于检测的方法和用于诊断甲型流感病毒的H5和N1基因的存在的试剂盒。 本发明特别适用于诊断领域。
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