公开(公告)号：US20230030539A1

公开(公告)日：2023-02-02

申请号：US17782290

申请日：2020-12-04

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Elie Fux ,  Sandra Gonzalez Maldonado ,  Michael Herold

IPC: G16B40/10 ,  G01N33/68 ,  G16H50/20 ,  G16H10/40

Abstract: The invention relates to a method of analyzing the metabolic content of a biological sample comprising: i) providing one or more samples of extracted metabolites from the biological sample; ii) performing a chromatography coupled mass spectrometry analysis of the extracted metabolites to generate a full raw data set for full scan ions; iii) generating a full data cluster set from the full raw data set obtained in step ii) by grouping full scan ions according to isotope and adduct values; iv) performing a tandem mass spectrometry analysis of the extracted metabolites with a plurality of mass selection windows to generate a raw SWATH® data set for fragment ions; v) generating a SWATH® data cluster set from the raw SWATH® data set obtained in step iv) by grouping fragment ions according to retention time and mass values; vi) aligning the SWATH® data cluster set with the full data cluster set to generate characteristic profile for each extracted metabolite; vii) comparing the data using R characteristic profile of each extracted metabolite obtained in step vi) with a reference library of characteristic profiles of metabolites to provide the metabolic content of the biological sample.

公开(公告)号：US20190004035A1

公开(公告)日：2019-01-03

申请号：US16064672

申请日：2016-12-21

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Elie Fux ,  Martin Dostler ,  Philipp Ternes

IPC: G01N33/50 ,  G01N1/40

Abstract: Provided herein is a method for determining a metabolic state of a plant or part thereof comprising a) rapid evaporating a multitude of metabolites of said plant or part thereof; b) determining the amount of at least one metabolite characteristic of said metabolic state; and c) thereby, determining a metabolic state of a plant thereof. Further provided is a method for in vivo determining a metabolite distribution in a plant or part thereof comprising a) in vivo rapid evaporating at least one metabolite of interest in at least a first and a second location of said plant or part thereof; b) determining the amounts of at least one metabolite at said first and a second location; and, c) thereby, in vivo determining metabolite distribution in a plant or part thereof. Moreover, provided are devices, data collections, and uses relating to the aforesaid methods.