公开(公告)号：US20220340918A1

公开(公告)日：2022-10-27

申请号：US17620775

申请日：2020-06-17

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Maarten Hendrik Stuiver ,  Doreen Schachtschabel ,  Birgit Hoff ,  Tobias Mentzel ,  Isabella Siepe ,  Eckhard Thines ,  Stefan Jacob ,  Michael Becker ,  Alexander Yemelin

IPC: C12N15/82 ,  C12N15/87 ,  C12N15/80

Abstract: The present invention provides to a method for the introducing polynucleotide molecules into fungal spores comprising exposing a mixture of fungal spores and magnetic nanoparticles carrying the polynucleotide to a magnet and/or a magnetic field. The present invention also provides a method for transformation a fungus comprising the steps of the method steps disclosed herein and allowing the integration of said polynucleotide molecules into the genome of said spores, thereby transforming the spore. The present invention also provides a system for delivery of nucleic acids to fungal spores comprising magnetic nanoparticles loaded with nucleic acids, a kit for transformation of fungal spores with a polynucleotide comprising MNPs loaded with the polynucleotide and fungal spores as well as a composition comprising MNPs loaded with nucleic acid molecules and fungal spores.

公开(公告)号：US20170183675A1

公开(公告)日：2017-06-29

申请号：US15449554

申请日：2017-03-03

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Josef Martin Kuhn ,  Holger Schultheiss ,  Julia Verena Hartig ,  Linda Patricia Loyall ,  Elke Duwenig ,  Marc Saric ,  Maarten Hendrik Stuiver ,  Oliver Oswald

IPC: C12N15/82 ,  A01H4/00

CPC classification number: C12N15/8216 ,  C12N15/8222 ,  C12N15/8223 ,  C12N15/8225 ,  C12N15/8239 ,  C12N15/8241

Abstract: Isolated nucleic acid molecules capable of regulating expression in plants, as well as expression [cassettes, vectors and transgenic plants comprising the same are provided.