公开(公告)号：US12209108B2

公开(公告)日：2025-01-28

申请号：US16956058

申请日：2018-12-12

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Meghan K. Wyrick ,  Min Xia ,  Janna Slessareva

IPC: C07K1/36 ,  A23J1/00 ,  A23J3/16 ,  C07K1/14

Abstract: The present invention provides the method and means for an efficient isolation of membrane-bound proteins from biological samples, e.g. in samples from raw or processed plant material, preferably defatted plant seed meal such as canola meal. The biological sample can be highly processed, e.g. by applying high temperature, pressure, or a chemical treatment and can be derived from seed matrices as well as other typical plant tissues for example seed, grain, leaf, root, or pollen. The invention comprises the provision of a novel extraction buffer (MEB) and its application in the method of the invention, wherein the buffer has a strong alkaline pH of 10 to 12.5 and comprises a soluble concentration of detergent at a level of 0.5% to 5%.

公开(公告)号：US20250154196A1

公开(公告)日：2025-05-15

申请号：US19027544

申请日：2025-01-17

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Meghan K. Wyrick ,  Min Xia ,  Janna Slessareva

IPC: C07K1/14 ,  A23J1/00 ,  A23J3/16 ,  C07K1/36

Abstract: The present invention provides the method and means for an efficient isolation of membrane-bound proteins from biological samples, e.g. in samples from raw or processed plant material. The biological sample can be highly processed, e.g. by applying high temperature, pressure, or a chemical treatment and can be derived from seed matrices as well as other typical plant tissues for example seed, grain, leaf, root, or pollen. The invention comprises the provision of a novel extraction buffer (MEB) and its application in the method of the invention.

公开(公告)号：US20200369717A1

公开(公告)日：2020-11-26

申请号：US16956058

申请日：2018-12-12

Applicant: BASF PLANT SCIENCE COMPANY GMBH

Inventor： Meghan K. Wyrick ,  Min Xia ,  Janna Slessareva

IPC: C07K1/14 ,  A23J1/00 ,  A23J3/16 ,  C07K1/36

Abstract: The present invention provides the method and means for an efficient isolation of membrane-bound proteins from biological samples, e.g. in samples from raw or processed plant material, preferably defatted plant seed meal such as canola meal. The biological sample can be highly processed, e.g. by applying high temperature, pressure, or a chemical treatment and can be derived from seed matrices as well as other typical plant tissues for example seed, grain, leaf, root, or pollen. The invention comprises the provision of a novel extraction buffer (MEB) and its application in the method of the invention, wherein the buffer has a strong alkaline pH of 10 to 12.5 and comprises a soluble concentration of detergent at a level of 0.5% to 5%.