公开(公告)号：US20150150160A1

公开(公告)日：2015-05-28

申请号：US14368662

申请日：2013-01-04

Applicant: BASF Plant Science Company GmbH

Inventor： Rocio Sanchez-Fernandez ,  Christian Biesgen ,  Holger Puchta ,  Nadine Roth ,  Friedrich Fauser ,  Michael Pacher

IPC: C12N15/90

CPC classification number: C12N15/902 ,  C12N15/8213

Abstract: The present invention relates to methods to modify at least one target locus in a plant cell, comprising, providing a plant cell with one or more target loci and one or more donor loci, followed by induction of homologous recombination between homologous regions of at least one target locus and at least one donor locus by at least one rare cleaving nuclease. The present invention related also to target loci, donor loci and nuclease loci used in these methods, and plant cells, plants and plant parts comprising these target loci, donor loci, nuclease loci and/or the recombined loci.

Abstract translation: 本发明涉及修饰植物细胞中至少一个靶基因座的方法，其包括向植物细胞提供一个或多个靶基因座和一个或多个供体基因座，随后在至少一个靶基因座的同源区域之间诱导同源重组 靶基因座和至少一个供体位点通过至少一种罕见的切割核酸酶。 本发明还涉及这些方法中使用的靶基因座，供体基因座和核酸酶基因座，以及包含这些靶基因座，供体基因座，核酸酶位点和/或重组基因座的植物细胞，植物和植物部分。