公开(公告)号：US20230212532A1

公开(公告)日：2023-07-06

申请号：US17759954

申请日：2021-02-01

申请人： BAYER AKTIENGESELLSCHAFT ,  BAYER PHARMA AKTIENGESELLSCHAFT

发明人： Oliver KENSCH ,  Kai THEDE ,  Petra HELFRICH ,  Lilly SKALDEN ,  Ludwig ZORN ,  Sabine TRENNER ,  Jens BURMEISTER ,  Nils KRETSCHMANN ,  Florian RICHTER ,  Wayne COCO ,  Marcus LUDWIG ,  Dalia BULUT ,  Frank BERENDES ,  Jens PILLING ,  Jakob WAGNER ,  Ruben LINNHOFF

IPC分类号： C12N9/02 ,  C12P33/06

CPC分类号： C12N9/0071 ,  C12P33/06 ,  C12Y114/14001

摘要： The present invention relates to novel recombinant Bacillus megaterium cytochrome P450-monooxygenase (P450-BM3) variants for the C19 hydroxylation of steroids and derivatives thereof or for improved BM3 protein expression. In particular, the present invention also relates to methods and processes using P450-BM3 variants for the production of estrone and estradiol. The invention further relates to nucleotide sequences, constructs and vectors for the expression of these P450-BM3 variants