公开(公告)号：US20140259213A1

公开(公告)日：2014-09-11

申请号：US13789533

申请日：2013-03-07

Applicant: BAYER CROPSCIENCE AG

Inventor： Gudrun Lange ,  Fabien Poree ,  Bernd Laber ,  Joerg Freigang ,  Arno Schultz

IPC: C12N15/82 ,  C12N9/02

CPC classification number: C12N15/8274 ,  C12N9/0008 ,  C12Y102/03013

Abstract: The present invention relates to an isolated nucleic acid comprising a nucleotide sequence encoding a mutated HPPD protein, wherein said mutated HPPD protein has HPPD activity, wherein in said mutated HPPD protein at least one amino acid has been replaced so that the resulting amino acid sequence comprises at least one amino acid selected from certain amino acids at specific positions important for conferring an increased HPPD inhibitor tolerance. The present invention also relates to proteins encoded by the nucleic acid of the invention, to chimeric genes, plant cells comprising the nucleic acid of the invention operably linked to a plant-expressible promoter and optionally a transcription termination and polyadenylation region, plants essentially consisting of the plant cells of the invention and methods of obtaining transgenic plants.

Abstract translation: 本发明涉及分离的核酸，其包含编码突变的HPPD蛋白的核苷酸序列，其中所述突变的HPPD蛋白具有HPPD活性，其中在所述突变的HPPD蛋白中至少一个氨基酸被替换，使得得到的氨基酸序列包含 至少一种选自特定位置的某些氨基酸的氨基酸，其对于赋予增加的HPPD抑制剂耐受性是重要的。 本发明还涉及由本发明的核酸编码的蛋白质，嵌合基因，包含与植物可表达的启动子和任选的转录终止和多聚腺苷酸化区域可操作地连接的本发明核酸的植物细胞，基本上由 本发明的植物细胞和获得转基因植物的方法。