公开(公告)号：US20230366023A1

公开(公告)日：2023-11-16

申请号：US18246959

申请日：2021-10-09

Applicant: BGI Shenzhen Co., Ltd.

Inventor： Yan Chen ,  Handong Li ,  Yongwei Zhang

IPC: C12Q1/6874

CPC classification number: C12Q1/6874

Abstract: Disclosed herein are methods and compositions for corresponding positions on an array with differently labeled affinity reagents immobilized at the positions. A first and a second affinity reagents are immobilized on at least some of the first and second positions, respectively. The first affinity reagent and the second affinity reagent are associated with a first luciferase polypeptide and a second luciferase polypeptide, respectively. The first luciferase polypeptide does not cross react with the second substrate and the second luciferase polypeptide does not cross react with the first substrate. The method further comprises contacting the array with the first substrate, which reacts with the first luciferase polypeptide and detecting the first luminescent signal and detecting the second luminescent signal at positions, thereby corresponding positions on an array with the first affinity reagent or the second affinity reagent immobilized at the positions.

公开(公告)号：US20210040556A1

公开(公告)日：2021-02-11

申请号：US16987092

申请日：2020-08-06

Applicant: BGI Shenzhen Co., Ltd.

Inventor： Yan Chen ,  Handong Li

IPC: C12Q1/6874 ,  C07D401/12 ,  G01N21/76

Abstract: Acridinium ester-containing compounds are provided herein. Also provided herein are chemiluminescence-based one-color sequencing methods and methods of using the acridinium ester-containing compounds in chemiluminescence-based one-color sequencing.