公开(公告)号：US11098298B2

公开(公告)日：2021-08-24

申请号：US16400520

申请日：2019-05-01

申请人： BIOMÉRIEUX DEUTSCHLAND GMBH

发明人： Bernd Buchberger ,  Holger Grallert ,  Sonja Molinaro

IPC分类号： C12N9/64 ,  G01N33/579

摘要： The present invention provides a novel method for the recombinant production of Factor C protein from horseshoe crab using a parasitic protozoan expressing the Factor C protein. In particular, the present invention provides a parasitic protozoan host cell harbouring a polynucleotide encoding horseshoe crab Factor C protein, and a method for producing Factor C protein comprising culturing said parasitic protozoan host cell under conditions such that the cells express the horseshoe crab Factor C protein. Furthermore, the present invention provides recombinant Factor C protein produced by the novel method and its use in the detection and/or removal of endotoxin.