公开(公告)号：US20140349875A1

公开(公告)日：2014-11-27

申请号：US14330535

申请日：2014-07-14

Applicant: BIOMERIEUX

Inventor： Juliette GIMENEZ ,  Cecile MONTGIRAUD ,  Francois MALLET

IPC: C12Q1/68

CPC classification number: C12Q1/6886 ,  C12Q1/686 ,  C12Q1/702 ,  C12Q2535/125 ,  C12Q2600/154 ,  C12Q2600/158

Abstract: A method for in vitro diagnosis of testicular cancer includes (i) obtaining a biological sample from a patient suspected of having testicular cancer, (ii) performing an assay to determine the methylation status of CpG dinucleotides in a genomic DNA target sequence, the DNA target sequence being the 5′ LTR U3 promoter sequence of the ERVWE1 locus, optionally further including an activator sequence directly upstream of the 5′ LTR U3 promoter sequence, and (iii) diagnosing the patient with testicular cancer when the DNA target sequence is hypomethylated as compared to a methylation status indicative of the absence of testicular cancer.

Abstract translation: 用于体外诊断睾丸癌的方法包括：（i）从疑似患有睾丸癌的患者获得生物样品，（ii）进行测定以确定基因组DNA靶序列中CpG二核苷酸的甲基化状态，DNA靶标 序列是ERVWE1基因座的5'LTR U3启动子序列，任选地还包括直接在5'LTR U3启动子序列上游的激活剂序列，和（iii）当DNA靶序列被低甲基化时诊断患有睾丸癌的患者， 指示缺乏睾丸癌的甲基化状态。