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1.发明授权Methods for determining enzymatic activity comprising heating and agitation of closed volumes 有权用于测定酶活性的方法，包括加热和搅拌封闭体积公开(公告)号：US09284596B2
公开(公告)日：2016-03-15
申请号：US14180161
申请日：2014-02-13
CPC分类号： C12Q1/40 , B01L3/5085 , B01L7/00 , B01L2200/025 , B01L2200/12 , B01L2300/0609 , B01L2300/0829 , B01L2300/1805
摘要： Methods for determining thermophilic enzymatic activity include heating a substrate solution in a plurality of closed volumes to a predetermined reaction temperature. Without opening the closed volumes, at least one enzyme is added, substantially simultaneously, to the closed volumes. At the predetermined reaction temperature, the closed volumes are agitated and then the activity of the at least one enzyme is determined. The methods are conducive for characterizing enzymes of high-temperature reactions, with insoluble substrates, with substrates and enzymes that do not readily intermix, and with low volumes of substrate and enzyme. Systems for characterizing the enzymes are also disclosed.
摘要翻译： 用于测定嗜热酶活性的方法包括将多个封闭体积的底物溶液加热至预定的反应温度。 在不打开封闭体积的情况下，至少一种酶基本同时地加入到封闭体积中。 在预定的反应温度下，搅拌闭合体积，然后确定至少一种酶的活性。 这些方法有利于表征高温反应的酶，不溶性底物，不容易混合的底物和酶，以及低体积的底物和酶。 还公开了用于表征酶的系统。
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2.发明申请SYSTEMS AND CONDUCTIVE STRUCTURES FOR DETERMINING ENZYMATIC ACTIVITY AND METHODS OF FORMATION 审中-公开用于确定酶活性的系统和导电结构及其形成方法公开(公告)号：US20160108455A1
公开(公告)日：2016-04-21
申请号：US14976124
申请日：2015-12-21
CPC分类号： C12Q1/40 , B01L3/5085 , B01L7/00 , B01L2200/025 , B01L2200/12 , B01L2300/0609 , B01L2300/0829 , B01L2300/1805
摘要： Methods for determining thermophilic enzymatic activity include heating a substrate solution in a plurality of closed volumes to a predetermined reaction temperature. Without opening the closed volumes, at least one enzyme is added, substantially simultaneously, to the closed volumes. At the predetermined reaction temperature, the closed volumes are agitated and then the activity of the at least one enzyme is determined. The methods are conducive for characterizing enzymes of high-temperature reactions, with insoluble substrates, with substrates and enzymes that do not readily intermix, and with low volumes of substrate and enzyme. Systems for characterizing the enzymes are also disclosed.
摘要翻译： 用于测定嗜热酶活性的方法包括将多个封闭体积的底物溶液加热至预定的反应温度。 在不打开封闭体积的情况下，至少一种酶基本同时地加入到封闭体积中。 在预定的反应温度下，搅拌闭合体积，然后确定至少一种酶的活性。 这些方法有利于表征高温反应的酶，不溶性底物，不容易混合的底物和酶，以及低体积的底物和酶。 还公开了用于表征酶的系统。
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公开(公告)号：US20150225765A1
公开(公告)日：2015-08-13
申请号：US14180161
申请日：2014-02-13
IPC分类号： C12Q1/40
CPC分类号： C12Q1/40 , B01L3/5085 , B01L7/00 , B01L2200/025 , B01L2200/12 , B01L2300/0609 , B01L2300/0829 , B01L2300/1805
摘要： Methods for determining thermophilic enzymatic activity include heating a substrate solution in a plurality of closed volumes to a predetermined reaction temperature. Without opening the closed volumes, at least one enzyme is added, substantially simultaneously, to the closed volumes. At the predetermined reaction temperature, the closed volumes are agitated and then the activity of the at least one enzyme is determined. The methods are conducive for characterizing enzymes of high-temperature reactions, with insoluble substrates, with substrates and enzymes that do not readily intermix, and with low volumes of substrate and enzyme. Systems for characterizing the enzymes are also disclosed.
摘要翻译： 用于测定嗜热酶活性的方法包括将多个封闭体积的底物溶液加热至预定的反应温度。 在不打开封闭体积的情况下，至少一种酶基本同时地加入到封闭体积中。 在预定的反应温度下，搅拌闭合体积，然后确定至少一种酶的活性。 这些方法有利于表征高温反应的酶，不溶性底物，不容易混合的底物和酶，以及低体积的底物和酶。 还公开了用于表征酶的系统。
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