公开(公告)号：US10703775B2

公开(公告)日：2020-07-07

申请号：US16080453

申请日：2017-03-01

Applicant: Bayer CropScience LP

Inventor： Chad Kimmelshue ,  Yan Li ,  Colleen S. Taylor ,  Hong Zhu

IPC: C07K1/36 ,  C07K1/30 ,  C12P21/02 ,  C07K7/00 ,  C12P19/04 ,  C07K7/64

Abstract: The present invention provides a method of enriching a lipopeptide in a microbial cell culture, the method comprising mixing an amphiphilic sulfonate and/or an amphiphilic sulfate with the cell culture to induce the formation of aggregates containing the lipopeptide, centrifuging the cell culture to generate a supernatant fraction and a pellet fraction, separating the pellet fraction from the supernatant fraction, and mixing the pellet fraction with a polyoxyethylene glycol alkyl ether to release the lipopeptide from the aggregates. Also provided is a method of purifying an exopolysaccharide from a microbial cell culture, the method comprising mixing an amphiphilic sulfonate and/or an amphiphilic sulfate with the cell culture to induce the formation of aggregates, centrifuging the cell culture to generate a supernatant fraction and a pellet fraction, separating the supernatant fraction from the pellet fraction, adding alcohol to the supernatant fraction to precipitate the exopolysaccharide; and removing the precipitated exopolysaccharide from the supernatant fraction.