公开(公告)号：US3964971A

公开(公告)日：1976-06-22

申请号：US517797

申请日：1974-10-24

申请人： Bela Johan ,  Laszlo Szemler ,  Janos Fulop ,  Tamas Szontagh ,  Emilia Simonovits nee Czink ,  Judit Bekes nee Erdos ,  Laszlo Kuti ,  Robert Toros ,  Denes Szekely ,  Laszlo Szabo ,  Karoly Seitz ,  Tade Vajda ,  Erzsebet Kovacs nee Komoroczy ,  Aniko Hargital nee Franyo ,  Karoly Polinszky

发明人： Bela Johan ,  Laszlo Szemler ,  Janos Fulop ,  Tamas Szontagh ,  Emilia Simonovits nee Czink ,  Judit Bekes nee Erdos ,  Laszlo Kuti ,  Robert Toros ,  Denes Szekely ,  Laszlo Szabo ,  Karoly Seitz ,  Tade Vajda ,  Erzsebet Kovacs nee Komoroczy ,  Aniko Hargital nee Franyo ,  Karoly Polinszky

IPC分类号： C07H23/00 ,  C12P5/02 ,  C12P19/42 ,  C12D5/06

CPC分类号： C12P19/42 ,  C12P5/023 ,  Y02E50/343 ,  Y10S435/801 ,  Y10S435/813

摘要： The invention relates to a method for increasing the vitamin B.sub.12 production of fermentation processes performed in a known way with a mixed population of mesophilic methane-producing bacteria under anaerobic conditions. According to the invention in an enrichment period of preferably 4 to 7 days a nutrient concentrate containing mainly inorganic ammonium compounds as nitrogen source and mainly methanol as carbon source and having a N:C weight ratio of 1:10 to 1:20, preferably 1:11 to 1:15 is added in daily portions to a fermentation broth obtained from a usual vitamin B.sub.12 fermentation process and containing living bacterium populations so as to increase the total concentration of assimilable nitrogen by a factor of maximum 4 until the end of the enrichment period, and thereafter the fermentation is terminated and the obtained fermentation broth with an increased vitamin B.sub.12 content is processed, or the fermentation is continued by periodically removing a portion of the fermentation broth and supplementing it with a same volume of fresh nutrient medium containing mainly inorganic ammonium compounds as nitrogen source and mainly methanol as carbon source and having a N:C weight ratio of 1:10 to 1:20, preferably 1:11 to 1:15.By this process fermentation broths with as high vitamin B.sub.12 concentration as 40.000 mcg./l. can be obtained.

摘要翻译： 本发明涉及一种增加在厌氧条件下用嗜温甲烷生产菌混合种群以已知方法进行的发酵过程的维生素B12产生的方法。 根据本发明，优选4至7天的富集期间，主要以无机铵化合物为氮源，主要为甲醇为碳源，且N:C重量比为1:10至1:20，优选1 ：将11〜1:15加入到通常的维生素B12发酵方法得到的发酵液中，并含有活细菌种群，以将可吸收氮的总浓度提高到4倍直至浓缩结束为止 期间，然后终止发酵，并且处理具有增加的维生素B12含量的所得发酵液，或者通过周期性地除去一部分发酵液并补充相同体积的主要含有无机物的新鲜营养培养基来继续发酵 铵化合物作为氮源，主要是甲醇作为碳源，N:C重量比为1:10至1:20， 优选1:11至1:15。