公开(公告)号：US20220326217A1

公开(公告)日：2022-10-13

申请号：US17640548

申请日：2020-09-04

申请人： Benaroya Research Institute at Virginia Mason

发明人： Eric E. Wambre ,  Justine J. Calise

IPC分类号： G01N33/50 ,  G01N33/68 ,  A61P37/08

摘要： Provided are methods and compositions for labeling an allergen-specific pathogenic CD4+ T-cell. The method can comprise contacting a cell population comprising CD4+ T cells with a suspected allergen to provide a challenged cell population, contacting the challenged cell population, or a subpopulation thereof, with a first molecule that specifically binds to a biomarker for an allergen-specific pathogenic T cell, wherein binding of the first molecule to the biomarker on a CD4+ cell indicates the cell is an allergen-specific pathogenic CD4+ T cell, and detecting binding of the first molecule to a CD4+ cell, wherein binding to the cell indicates the cell is an allergen-specific pathogenic CD4+ T cell. The method is applicable to monitoring the presence of allergen-specific pathogenic CD4+ T cells and/or efficacy of immunotherapy for allergies in a subject.