公开(公告)号：US08088595B2

公开(公告)日：2012-01-03

申请号：US12660884

申请日：2010-03-05

申请人： Bennett L. Ibey ,  Vamsi K. Yadavalli ,  Rebecca M. Rounds ,  Hope T. Beier ,  Gerard L. Cote ,  Michael V. Pishko

发明人： Bennett L. Ibey ,  Vamsi K. Yadavalli ,  Rebecca M. Rounds ,  Hope T. Beier ,  Gerard L. Cote ,  Michael V. Pishko

IPC分类号： C12Q1/54

CPC分类号： C12Q1/54 ,  G01N33/53 ,  G01N33/54366

摘要： This disclosure relates to a method of measuring a glucose concentration metric or a glucose metric in a patient by contacting an implantable glucose-sensing device with a test sample, which may be in the patient, under conditions that permit a sugar-binding molecule and a functionalized polymer or nano-particle ligand present throughout the matrix of a hydrogel to interact in a glucose-dependent manner to produce an optical signal resulting from quenching of a first fluorophore linked to the ligand or sugar-binding molecule and having a fluorescent emission spectrum quenched upon binding or release of glucose. Next the first fluorophore may be excited with light of a certain wavelength. Then at least one wavelength of light in the glucose-dependent optical signal from the fluorophore may be detected with a detector to produce a detected light signal, which may be processed to produce a glucose metric, such as a glucose concentration metric.

摘要翻译： 本公开涉及一种通过使可植入葡萄糖感测装置与可能在患者体内的测试样品在允许糖结合分子和 存在于水凝胶整个基质中的官能化聚合物或纳米颗粒配体以葡萄糖依赖性方式相互作用以产生由与配体或糖结合分子连接并具有荧光发射光谱淬灭的第一荧光团猝灭而产生的光学信号 葡萄糖结合或释放时。 接下来，可以用一定波长的光激发第一荧光团。 然后可以用检测器检测来自荧光团的葡萄糖依赖性光信号中的至少一个波长的光，以产生检测到的光信号，其可以被处理以产生葡萄糖度量，例如葡萄糖浓度度量。

公开(公告)号：US20100222657A1

公开(公告)日：2010-09-02

申请号：US12660884

申请日：2010-03-05

申请人： Bennett L. Ibey ,  Vamsi K. Yadavalli ,  Rebecca M. Rounds ,  Hope T. Beier ,  Gerard L. Cote ,  Michael V. Pishko

发明人： Bennett L. Ibey ,  Vamsi K. Yadavalli ,  Rebecca M. Rounds ,  Hope T. Beier ,  Gerard L. Cote ,  Michael V. Pishko

IPC分类号： A61B5/1459 ,  A61K49/00 ,  A61P3/10

CPC分类号： C12Q1/54 ,  G01N33/53 ,  G01N33/54366

摘要： This disclosure relates to systems, devices, and methods of sensing an analyte. An implantable sensor may be contacted with a test sample under conditions that permit a binding protein and a ligand of the sensor to interact in an analyte-dependent manner to produce an analyte-dependent signal, and (b) detecting the analyte-dependent signal with a detector. A binding protein may reversibly bind an analyte and/or a ligand. A binding protein may have a higher binding affinity for an analyte than for a ligand. A binding protein and a ligand may each include a fluorophore, the absorption and/or emission properties of which may change in an analyte-dependent manner. A binding protein and/or a ligand may be bound to an active or inactive substrate. Some embodiments of systems, devices, and methods may be practiced in vitro, in situ, and/or in vivo. Systems and/or devices of the disclosure may be configured to be wearable.

摘要翻译： 本公开涉及感测分析物的系统，装置和方法。 在允许结合蛋白和传感器的配体以分析物依赖的方式相互作用以产生分析物相关信号的条件下，可植入传感器可以与测试样品接触，并且（b）用分析物依赖的信号检测 检测器 结合蛋白质可以可逆地结合分析物和/或配体。 结合蛋白质可能对分析物具有比配体更高的结合亲和力。 结合蛋白和配体可以各自包括荧光团，其吸收和/或发射性质可以以分析物依赖的方式改变。 结合蛋白和/或配体可以与活性或非活性底物结合。 系统，装置和方法的一些实施方案可以在体外，原位和/或体内实践。 本公开的系统和/或设备可以被配置为可穿戴的。

公开(公告)号：US07704704B2

公开(公告)日：2010-04-27

申请号：US11529280

申请日：2006-09-28

申请人： Bennett L. Ibey ,  Vamsi K. Yadavalli ,  Rebecca M. Rounds ,  Hope T. Beier ,  Gerard L. Cote ,  Michael V. Pishko

发明人： Bennett L. Ibey ,  Vamsi K. Yadavalli ,  Rebecca M. Rounds ,  Hope T. Beier ,  Gerard L. Cote ,  Michael V. Pishko

IPC分类号： C12Q1/54

CPC分类号： C12Q1/54 ,  G01N33/53 ,  G01N33/54366

摘要： This disclosure relates to systems, devices, and methods of sensing an analyte. An implantable sensor may be contacted with a test sample under conditions that permit a binding protein and a ligand of the sensor to interact in an analyte-dependent manner to produce an analyte-dependent signal, and (b) detecting the analyte-dependent signal with a detector. A binding protein may reversibly bind an analyte and/or a ligand. A binding protein may have a higher binding affinity for an analyte than for a ligand. A binding protein and a ligand may each include a fluorophore, the absorption and/or emission properties of which may change in an analyte-dependent manner. A binding protein and/or a ligand may be bound to an active or inactive substrate. Some embodiments of systems, devices, and methods may be practiced in vitro, in situ, and/or in vivo. Systems and/or devices of the disclosure may be configured to be wearable.

摘要翻译： 本公开涉及感测分析物的系统，装置和方法。 在允许结合蛋白和传感器的配体以分析物依赖的方式相互作用以产生分析物相关信号的条件下，可植入传感器可以与测试样品接触，并且（b）用分析物依赖的信号检测 检测器 结合蛋白质可以可逆地结合分析物和/或配体。 结合蛋白质可能对分析物具有比配体更高的结合亲和力。 结合蛋白和配体可以各自包括荧光团，其吸收和/或发射性质可以以分析物依赖的方式改变。 结合蛋白和/或配体可以与活性或非活性底物结合。 系统，装置和方法的一些实施方案可以在体外，原位和/或体内实践。 本公开的系统和/或设备可以被配置为可穿戴的。