公开(公告)号：US20050130262A1

公开(公告)日：2005-06-16

申请号：US10484380

申请日：2002-07-12

申请人： Bertrand Lambolez ,  Nathalie Gibelin ,  Gaelle Bourout ,  Ludovic Tricoire ,  Olivier Courjean ,  Keisuke Tsuzuki ,  Jean Rossier

发明人： Bertrand Lambolez ,  Nathalie Gibelin ,  Gaelle Bourout ,  Ludovic Tricoire ,  Olivier Courjean ,  Keisuke Tsuzuki ,  Jean Rossier

IPC分类号： C07K14/435 ,  C12N15/12 ,  C07H21/04 ,  C12N5/02

CPC分类号： C07K14/43595

摘要： Mutated photoproteins derived from isolated jellyfish photoproteins, characterised in that they exhibit a thermostability higher than that of the photoproteins from which they are derived, and are called thermostable mutated photoproteins, and/or a luminescence duration longer than that of the photoproteins from which they are derived, and are called persistent mutated photoproteins. The invention also concerns the use of said mutated photoproteins in methods for detecting in vitro molecules in a biological sample, methods for detecting compounds with enzymatic activity in a biological sample, or methods for detecting intracellular calcium variations.

摘要翻译： 衍生自分离的水母光蛋白的突变的光蛋白，其特征在于它们的热稳定性高于其衍生的光蛋白的热稳定性，并且被称为耐热突变的光蛋白，和/或发光持续时间长于它们的光蛋白的发光持续时间 衍生，被称为持续突变的光蛋白。 本发明还涉及所述突变的光蛋白在用于检测生物样品中的体外分子的方法中的用途，用于检测生物样品中具有酶活性的化合物的方法或用于检测细胞内钙变化的方法。