公开(公告)号：US10261058B2

公开(公告)日：2019-04-16

申请号：US14592308

申请日：2015-01-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jeff Habel

IPC: G01N33/48 ,  G01N33/50 ,  G01N30/86 ,  G06F19/18 ,  G01N30/74 ,  G01N30/46

Abstract: One embodiment of the invention is directed to a method of analyzing liquid chromatography data. The method comprises collecting, by a data processing system, first volume fractions data from a first liquid chromatography column for a first absorbance wavelength of light λ1 from a first run of a liquid chromatography process on a mixture, wherein the first liquid chromatography column screens for a first characteristic of the mixture. The method further comprising, normalizing a first relative peak area for a first volume of a component of interest in the mixture for the first absorbance wavelength λ1 to obtain a first set of purity quotient values PQ1, collecting second volume fractions data from a second liquid chromatography column for a second absorbance wavelength of light λ2 from a second run of a liquid chromatography process on the mixture, wherein the second liquid chromatography column screens for a second characteristic of the mixture, normalizing a second relative peak area for the second volume of the component of interest in the mixture for the second absorbance wavelength λ2 to obtain a second set of purity quotient values PQ2, storing the values PQ1 and PQ2 in a memory, calculating a difference between values PQ1 and PQ2 for each volume fraction location of the first and second volumes to obtain a first set of purity quotient difference (“PQD”) values, displaying in a graphical display the first set of PQD values, and determining which volume fractions to pool together based on the display of the first set of PQD values.