公开(公告)号：US20130109807A1

公开(公告)日：2013-05-02

申请号：US13654802

申请日：2012-10-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Peter S. GAGNON

IPC: C07K1/20 ,  C08F26/10

CPC classification number: C07K16/065

Abstract: The present invention provides chromatography methods for removing a virucidal agent from a target protein such as an antibody. The method uses a hydrophobic, negatively charged mixed mode support that has high affinity for both the target protein and the virucidal agent. The method provides conditions that favor dissociation of the virucidal agent from the protein, allowing the virucidal agent to bind strongly to the support. The target protein is then eluted from the support under conditions such that the virucidal agent remains bound to the support.

Abstract translation: 本发明提供了从靶蛋白如抗体中除去杀病毒剂的层析方法。 该方法使用对靶蛋白和杀病毒剂具有高亲和力的疏水性带负电荷的混合模式载体。 该方法提供有利于从杀虫剂与蛋白质解离的条件，使得杀病毒剂与载体强烈结合。 然后在使得杀病毒剂保持与载体结合的条件下从载体上洗脱靶蛋白质。