公开(公告)号：US20140120085A1

公开(公告)日：2014-05-01

申请号：US14036969

申请日：2013-09-25

Applicant: BioNTech AG

Inventor： Özlem TÜRECI ,  Ugur SAHIN ,  Sandra SCHNEIDER ,  Gerd HELFTENBEIN ,  Volker SCHLÜTER ,  Dirk USENER ,  Philippe THIEL ,  Michael KOSLOWSKI

IPC: C07K14/47

CPC classification number: A61K39/39558 ,  A61K39/395 ,  A61K45/06 ,  C07K14/4748 ,  C07K16/28 ,  C07K16/30 ,  C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/156 ,  G01N33/53

Abstract: An isolated truncated desmoglein 4 (DSG4) polypeptide splice variant of the invention is characterized by an amino acid sequence that lacks a region encoded before exon 9 or beyond exon 10 of the DSG4 gene having the polynucleotide sequence of SEQ ID NO: 75. Also disclosed is a method of diagnosing a cancer, or monitoring the course thereof, in a patient. The method comprises detecting in a tissue sample of a patient the expression of a tumor-associated antigen comprising the extracellular domain of a DSG4 polypeptide encoded by a DSG4 gene having the polynucleotide sequence of SEQ ID NO: 75, or a truncated DSG4 polypeptide splice variant characterized by an amino acid sequence that lacks a region encoded before exon 9 or beyond exon 10 of the DSG4 gene.

Abstract translation: 本发明的分离的截短的脱骨髓蛋白4（DSG4）多肽剪接变体的特征在于缺乏在具有SEQ ID NO：75的多核苷酸序列的DSG4基因的外显子9或超出外显子10之前编码的区域的氨基酸序列。还公开了 是在患者中诊断癌症或监测其过程的方法。 该方法包括在患者的组织样品中检测包含由具有SEQ ID NO：75的多核苷酸序列的DSG4基因或截短的DSG4多肽剪接变体编码的DSG4多肽的细胞外结构域的肿瘤相关抗原的表达 其特征在于缺乏在DSG4基因的外显子9或超出外显子10之前编码的区域的氨基酸序列。