公开(公告)号：US20020051998A1

公开(公告)日：2002-05-02

申请号：US09733759

申请日：2000-12-08

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Claudia Schmidt-Dannert ,  Frances H. Arnold

IPC: G01N033/53 ,  C12P023/00 ,  C12N005/04 ,  C12N005/06

CPC classification number: C12N9/0004 ,  C12N15/1027 ,  C12N15/52 ,  C12P23/00

Abstract: The present invention relates to engineering new biosynthetic pathways into microorganisms, in particular biosynthetic carotenoid pathways. New and improved catalytic functions of metabolic pathways are created by, for example, site-specific mutation or gene shuffling techniques, to provide for efficient biosynthesis of carotenoids. By applying the described directed evolution techniques, almost any carotenoid could be produced, in a host cell, from one or a few sets of genes. In addition, the described techniques are useful for creating gene or protein libraries for new and uncharacterized carotenoids.

Abstract translation: 本发明涉及将新的生物合成途径工程化成微生物，特别是生物合成类胡萝卜素途径。 通过例如位点特异性突变或基因改组技术产生新的和改进的代谢途径的催化功能，以提供类胡萝卜素的有效生物合成。 通过应用所描述的定向进化技术，可以在宿主细胞中从一组或几组基因产生几乎任何类胡萝卜素。 此外，所描述的技术可用于产生新的和未表征的类胡萝卜素的基因或蛋白质文库。