公开(公告)号：US20010055786A1

公开(公告)日：2001-12-27

申请号：US09828599

申请日：2001-04-05

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Frances H. Arnold ,  John Joern ,  Takeshi Sakamoto ,  Ulrich Schwaneberg

IPC: C12Q001/26

CPC classification number: C12Q1/26 ,  C12Q1/32

Abstract: The present invention relates to screening methods for oxidation enzymes, particularly mono- and dioxygenases. According to the methods of the invention, a product of an oxidation reaction is converted into a phenol or a catechol, which is easily detected by a Gibbs assay. This conversion allows for a sensitive and efficient assay. Both high-throughput liquid-phase and solid-phase methods using these principles are provided. Also described are method for detecting phenolic ether-products and sulfhydryl products from oxidation reactions, also using a Gibbs assay.

Abstract translation: 本发明涉及氧化酶，特别是单加氧酶和双加氧酶的筛选方法。 根据本发明的方法，将氧化反应的产物转化成苯酚或邻苯二酚，其可以通过吉布斯测定容易地检测。 该转化允许敏感和有效的测定。 提供了使用这些原理的高通量液相和固相方法。 还描述了也使用吉布斯测定法从氧化反应中检测酚醚产物和巯基产物的方法。