公开(公告)号：US20170283773A1

公开(公告)日：2017-10-05

申请号：US15463737

申请日：2017-03-20

Applicant: CATHOLIC UNIVERSITY INDUSTRY ACADEMIC COOPERATION FOUNDATION

Inventor： So-Hyang CHUNG ,  Hyun Jung LEE

IPC: C12N5/079

CPC classification number: C12N5/0621 ,  C12N2501/415 ,  C12N2533/30

Abstract: Provided are methods for improving the proliferation and sternness of limbal stem cells (LSCs) by adding a Wnt inhibitor to a medium for limbal explant cultures.When human limbal explants are cultured according to the methods, the Wnt/β-catenin signaling is inhibited, resulting in improvement of LSC proliferation, and improvement in the sternness of LSCs, and thus the LSCs may be obtained in high yield. These effects may lead to a higher success rate of transplantation of a limbal explant outgrowth sheet into a LSCD patient.

公开(公告)号：US20190010454A1

公开(公告)日：2019-01-10

申请号：US16066389

申请日：2016-07-22

Applicant: CATHOLIC UNIVERSITY INDUSTRY ACADEMIC COOPERATION FOUNDATION

Inventor： So-Hyang CHUNG ,  Hyun Jung LEE ,  Kyung Yul SEO

IPC: C12N5/079 ,  A61L27/36

Abstract: The present invention relates to a method for culturing limbal tissues on an amniotic membrane slide scaffold, thereby enabling the proportion of limbal stem cells in a limbal tissue-derived epithelial cell sheet to be effectively increased, and the same to be cultured. According to the present invention, the proportion of limbal stem cells in in a limbal tissue-derived epithelial cell sheet can be stably and rapidly increased, and thus the success rate can be increased when in limbal tissue-derived epithelial cell sheets are transplanted into a patient with limbal stem cell deficiency.