公开(公告)号：US20200080073A1

公开(公告)日：2020-03-12

申请号：US16655228

申请日：2019-10-17

Applicant: CELLECTIS S. A.

Inventor： Alan D. King ,  Richard E. Walters ,  Stephen B. Deitz ,  Donald J. Rodis ,  Derin C. Walters

IPC: C12N13/00 ,  C12M1/42

Abstract: An electroporation apparatus and its novel chamber with inlet ports for mixing cells and exogenous material. The inlet ports are oriented in nonparallel to each other immediately adjacent at the same top comer of the first wall of the chamber. The mixing chamber comprises successive wall sections, two curved walls at its bottom; the first curved comer is on the same side of the chamber where the liquids enter the chamber, and directs the liquids to the second curved comer at the opposing side of the chamber, which in turn further redirects the mixing to the first curved corner. The direction of the liquid flow mixture change direction at least twice into the mixing chamber.