公开(公告)号：US10131913B2

公开(公告)日：2018-11-20

申请号：US14681351

申请日：2015-04-08

申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)

发明人： Marcel Mechali ,  Philippe Coulombe ,  Christelle Cayrou ,  Eric Rivals ,  Paulina Prorok

IPC分类号： C12N15/64 ,  C12N15/69

摘要： A method for initiating the replication of a deoxyribonucleic acid molecule includes inserting into the DNA at least one nucleic acid molecule representing a multicellular DNA replication origin. The replication origin contains at least nine nucleotides and contains at least three uninterrupted origin repeating elements (ORE), each ORE having the sequence N3GN4, wherein N3 is T or G and N4 is G or C. The method can confer autonomous replication properties to a non-self-replicating DNA molecule. A process for preparing a vector for use in said methods is also presented.

公开(公告)号：US20190093147A1

公开(公告)日：2019-03-28

申请号：US16155421

申请日：2018-10-09

申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)

发明人： Marcel Mechali ,  Philippe Coulombe ,  Christelle Cayrou ,  Eric Rivals ,  Paulina Prorok

IPC分类号： C12Q1/6806 ,  C12N15/10 ,  C12N15/79

摘要： The invention relates to a method for initiating the replication of a deoxyribonucleic acid molecule, said method comprising a step of inserting, into said deoxyribonucleic acid molecule, at least one nucleic acid molecule representing a multicellular DNA replication origin, the replication origin comprising at least nine nucleotides, the at least nine nucleotides consisting of at least three uninterrupted origin repeating elements (OGRE).