公开(公告)号：US20230085803A1

公开(公告)日：2023-03-23

申请号：US17992032

申请日：2022-11-22

申请人： CHUGAI SEIYAKU KABUSHIKI KAISHA

发明人： Masami Suzuki ,  Koichi Matsubara ,  Atsuhiko Kato ,  Chie Kato ,  Shinta Kobayashi ,  Yu Jau Chen ,  Masaki Yamazaki

IPC分类号： G01N33/50 ,  A01K67/027

摘要： An objective of the present invention is to provide non-human animal models of cancer pathology, which mimic the hierarchical organization, cancer progression process, or biological property of human cancer tissues, and uses thereof. To achieve the objective described above, first, the present inventors transplanted cells of NOG-established cancer lines into NOG mice and morphologically observed the resulting tissue organization. As a result, the non-human animal models were demonstrated to exhibit pathologies (the hierarchical organization, cancer progression process, or biological properties of the cancer cells) similar to that of human cancer. Specifically, the present inventors succeeded in preparing non-human animal models exhibiting pathologies more similar to a human cancer, and cell culture systems using NOG-established cancer cell lines where the in vitro cell morphology is more similar to that of human cancer.

公开(公告)号：US11965180B2

公开(公告)日：2024-04-23

申请号：US16994388

申请日：2020-08-14

申请人： CHUGAI SEIYAKU KABUSHIKI KAISHA

发明人： Tatsumi Yamazaki ,  Hisafumi Okabe ,  Shinta Kobayashi ,  Yu Jau Chen ,  Atsuhiko Kato ,  Masami Suzuki ,  Koichi Matsubara

IPC分类号： C12N5/095 ,  G01N33/50

CPC分类号： C12N5/0695 ,  G01N33/5011 ,  G01N33/5073

摘要： Provided is a cancer stem cell mass from which cells incapable of forming cancer are substantially removed and which has a characteristic property of reproducing a layered structure of a cancer tissue; a process for producing the cancer stem cell mass; and use of the cancer stem cell mass. A human cancer tissue was repeatedly grown in a NOG mouse, separated cancer cells from the grown cancer tissue, and tested and compared various cancer cell culture processes. As a result, a cancer stem cell composition which is homogeneous and is substantially free of the coexistence of cells capable of forming cancer and cells incapable of forming cancer in a mixed state can be produced successively by employing an attached culture process using a serum-free stem cell culture medium rather than a generally employed floating culture process.